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Spore breeding method for arsenic hyperaccumulator Pteris cretica

A technology for hyper-accumulation of plants and grass spores, applied in the fields of botanical equipment and methods, plant cultivation, horticulture, etc., can solve the problem of low seedling rate, achieve high seedling rate, promote growth and proliferation, and shorten germination time. Effect

Inactive Publication Date: 2017-05-10
YUNNAN GREEN LAND BIOLOGICAL TECH
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  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve the problem of low seedling rate in the spore propagation of the big-leaf wellhead edge grass, different sowing densities, different types and concentrations of nutrient solutions and hormone induction were designed to increase the germination rate of spores, the proliferation rate of prophyllites and the transformation rate of sporophytes , to achieve the effect of increasing the seedling rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of arsenic super-enriched plant big-leaf wellhead side grass seedling substrate, using a ratio of 60% peat soil and 40% perlite, mix well, divide into 10*10 seedling pots, and then use 800 times carbendazim Carry out matrix disinfection;

Embodiment 2

[0022] Sow the spore powder of the arsenic hyper-accumulative plant Dayejingkou Biancao. From July to September every year, go to the Dayejingkou Biancao female garden on the same day to collect robust and mature spore leaves, collect the spore powder, and weigh the spore powder at the same time 30-40mg / part, soak it with water, and sow it in the prepared matrix pot after 24 hours.

Embodiment 3

[0024] For arsenic super-enriched plants, the big-leaf wellhead side grass seedlings are managed and protected. The seedling pots that have been sown are watered thoroughly, placed in a seedling greenhouse, covered with plastic film, and cultivated with 75% sunshade nets. Regularly remove the film for ventilation, and regularly spray water to maintain humidity. During the cultivation period, the temperature in the shed is controlled between 15-30°C, and the humidity in the regular seedling cultivation pot is above 80%, and relatively dark light cultivation is maintained.

[0025] About 10-15 days after sowing, it was found that obvious spores germinated to form green filaments, and a large number of heart-shaped prothallus were formed around 30-35 days. At the same time, spray pH6, 1 / 2MS+20g / l sucrose+1g / lKH 2 PO 4 +0.2mg / lNAA proliferation nutrient solution 50ml / pot; after 60-70 days, the phylloplasma develops well, the phylloplasma covers the basin, the archegonium and sper...

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Abstract

The invention discloses a spore breeding method for arsenic hyperaccumulator Pteris cretica. The spore breeding method is implemented and completed by parts, such as a hybrid seedling matrix, a seedling pot, mature Pteris cretica spores and special management and protection and observation. The spore breeding method is capable of, according to the problems that the spore volume of the Pteris cretica spore seedling is small, the seeding rate is hard to be controlled, and the efficiency of forming sporophyte by gametophyte in the natural conditions is low, designing the spore breeding technology, solving the problem that the qualified seedling percentage of the Pteris cretica spore breeding in the natural conditions is low, and designing the complete breeding management technology in allusion to the special requirements of water, illumination and temperature in the breeding period of the Pteris cretica spore, and can be used as a method for breeding the arsenic hyperaccumulator Pteris cretica spores.

Description

technical field [0001] The invention relates to a heavy metal hyperaccumulation fern spore breeding method, in particular to a spore breeding method of the hyperaccumulator Pteris cretica var. The germination of the spores of the arsenic hyperaccumulative plant S. macrophyllum, the development of the gametophyte and the formation of new sporophytes, and the method of managing and protecting the sporophytes in the seedling stage, specifically relates to the spores of the arsenic hyperaccumulator plant S. macrophyllum Breeding method. Background technique [0002] The problem of heavy metal pollution in water and soil is one of the environmental problems that plague the world. In recent years, some special plants that can accumulate pollutants in large quantities in the ground—hyperaccumulators have become a hot spot in academic research. The use of hyperaccumulators to remove metal and metalloid pollution in soil and water environments—phytoremediation technology has attract...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
CPCA01G22/00
Inventor 童亚丽鲁明秀薛必猛宣艳辉
Owner YUNNAN GREEN LAND BIOLOGICAL TECH
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