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Method for preparing aescin of Chinese buckeye seeds

A kind of technology of aescin and aescin, which is applied in the field of preparation of aescin from aescin, can solve the problem of high loss rate of saponin components, and achieve the effect of less loss

Active Publication Date: 2017-04-26
BEIJING UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0004] In the above-mentioned extraction and purification methods of aescin, macroporous resin columns are used for separation and purification, and the loss rate of saponins is relatively large, and they still contain flavonoids, flavonoid glycosides and other impurities.

Method used

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  • Method for preparing aescin of Chinese buckeye seeds
  • Method for preparing aescin of Chinese buckeye seeds
  • Method for preparing aescin of Chinese buckeye seeds

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preparation example Construction

[0038] The preparation method of the aescin from the seeds of the present invention comprises: (1) an extraction step of the seeds of the sage, and (2) a purification step. In the purification step, a reverse-phase chromatographic column is used as a chromatographic column, and ethanol aqueous solutions of different concentrations are used for gradient elution, thereby obtaining an eluent enriched in aescin compounds. The preparation method of the present invention may also include: (3) the steps of concentrating and drying the eluate enriched in aescin compounds, so as to obtain solid aescin. In addition, the preparation method of the present invention may also include: (4) a step of washing the chromatographic column after the purification step is completed. The invention also provides a preparation method of sodium aescinate.

[0039] According to one embodiment of the present invention, the preparation method of the sage aescin includes the following steps:

[0040] (1) ...

Embodiment 1

[0067] The preparation method of the aescin of the present embodiment comprises the following steps:

[0068] (1) The 25vol% ethanol aqueous solution is used as solvent heating and reflux extracting 2 times of Sala seeds, the solvent consumption is successively 8 times and 6 times of the weight of Sala seeds, and the extraction time is 1.5 hours and 1 hour successively to obtain the extracted liquid;

[0069] (2) Concentrate and dry the extract to obtain an extract, dissolve the extract with 15vol% ethanol aqueous solution to obtain a loading sample containing about 40wt% aescin; reverse the loading sample Phase chromatographic column, adopt the 15vol% ethanol aqueous solution (first eluent) of 2 times column volume successively, the 60vol% ethanol aqueous solution (second eluent) of 3 times column volume elution, obtain the first elution successively Solution, the second eluent, collect the second eluent; collect the first eluent at the same time for verification;

[0070] ...

Embodiment 2

[0073] The second eluent was prepared by the method in Example 1, and the second eluent was concentrated and dried to obtain the solid Aescin.

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Abstract

The invention discloses a method for preparing aescin of Chinese buckeye seeds and a method for preparing sodium aescinate. The method for preparing aescin of Chinese buckeye seeds comprises the following steps: (1) extracting the Chinese buckeye seeds by adopting a 10-70vol% ethanol solution as a solvent, so as to obtain an extract; (2) preparing the extract into a fed sample, feeding the sample onto a reverse-phase chromatographic column, performing gradient elution by using a 8-25vol% ethanol solution and a 35-80vol% ethanol solution in sequence, and collecting the 35-80vol% ethanol solution, thereby obtaining an eluant. By adopting the method disclosed by the invention, impurities can be effectively removed, meanwhile the loss amount of aescin components is small, and in addition, decoloring can be achieved without additional activated carbon decoloring operation.

Description

technical field [0001] The invention relates to a preparation method of aescin from sage aescin. Background technique [0002] Salazi is a medicinal material that can soothe the liver and regulate qi, harmonize the stomach and relieve pain. It is used for stagnation of liver and stomach qi, chest and abdomen distension, and epigastric pain. The main active ingredient of Sala seed is aescin, which belongs to triterpenoid saponins, has anti-inflammatory, anti-exudation, restores capillary permeability, improves venous tension, improves blood circulation, dilates arteries, dilates coronary arteries, increases tolerance Hypoxic capacity, promote the recovery of brain function and so on. The marketed medicines prepared from saccharomyces aescin as raw materials include sodium aescinin for injection, compound sodium aescinate gel, etc. [0003] Most of the existing Aescins of Sala chinensis are extracted with ethanol aqueous solution of different concentrations to obtain the cru...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H15/256C07H1/08
CPCC07H1/08C07H15/256C07J63/008
Inventor 李军张倩屠鹏飞孙启泉王金铃赵云芳张静陈燕柑
Owner BEIJING UNIV OF CHINESE MEDICINE
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