A special compound microbial fertilizer for tobacco with the function of strengthening seedlings and promoting roots and its preparation method
A technology of compound microorganisms and tobacco is applied in the field of compound microorganism fertilizer special for tobacco and its preparation, which can solve the problems of insufficient systematic and in-depth research and failure to form large-scale promotion products, etc., and achieve the effects of improving yield quality, promoting root system development, and promoting growth.
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[0015] The first step is to prepare functional bacteria:
[0016] (1) The Bacillus thuringiensis bacterial liquid is prepared by the following method: inoculate the commercially available Bacillus thuringiensis test tube slant strains on the slant of the broth agar medium for slant activation culture, and then transfer the slant to the broth medium for seeding after 36 hours of activation culture Cultivate the seeds after 36 hours of inoculation and inoculate them into the broth medium at an inoculation amount of 15wt% to expand the culture until the amount of bacteria or spores in the culture solution reaches 4.0×10 9 Bacillus thuringiensis bacterium liquid; wherein, the condition of slope activation culture is: the cultivation temperature range is 35 ℃; the condition of seed cultivation and expanded cultivation is: the cultivation temperature range is 35 ℃, and the stirring speed is 250 rev / min; The preparation method of broth agar medium is: peptone 10g, beef extract 5g, gl...
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