Tissue culture media of Glossostigma elatinoides
A tissue culture medium, culture medium technology, applied in horticulture, botany equipment and methods, gardening tools/equipment and other directions, can solve the problems of quality and yield decline, planting benefit decline, virus accumulation, etc., to improve yield and quality, Improve the survival rate of carrier culture and the effect of strong vitality and tenaciousness
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Embodiment 1
[0013] The tissue culture medium of dwarf pearls includes three kinds of culture medium at different stages, and the raw materials and component concentrations of each culture medium are as follows,
[0014] The primary medium is: on the basis of ER medium, add potassium iodate 0.1mg / L, add 2,4-D 0.6mg / L, add paclobutrazol 1.2mg / L;
[0015] The subculture medium is: on the basis of MS medium, first adjust the concentration of ammonium nitrate to 1200mg / L, then add urea 100mg / L, add ABA3mg / L, add indole acetic acid 0.5mg / L, add glutamine Amide 5.0mg / L, add activated carbon 200mg / L;
[0016] The rooting medium is: in N 6 On the basis of the culture medium, 0.01 mg / L ammonium molybdate, 1.5 mg / L TDZ, 130 mg / L amylopectin were added, and the pH was adjusted to 7.0-7.2 with sodium hydroxide.
Embodiment 2
[0018] The tissue culture medium of dwarf pearls includes three kinds of culture medium at different stages, and the raw materials and component concentrations of each culture medium are as follows,
[0019] The primary medium is: on the basis of ER medium, add potassium iodate 0.5mg / L, add 2,4-D 0.8mg / L, add paclobutrazol 1.3mg / L;
[0020] The subculture medium is: on the basis of MS medium, first adjust the concentration of ammonium nitrate to 1300mg / L, then add urea 120mg / L, add ABA 3.5mg / L, add indole acetic acid 0.8mg / L, add Glutamine 5.5mg / L, add activated carbon 300mg / L;
[0021] The rooting medium is: in N 6 On the basis of the culture medium, 0.02 mg / L ammonium molybdate, 2.0 mg / L TDZ, 150 mg / L amylopectin were added, and the pH was adjusted to 7.2 with sodium hydroxide.
Embodiment 3
[0023] The tissue culture medium of dwarf pearls includes three kinds of culture medium at different stages, and the raw materials and component concentrations of each culture medium are as follows,
[0024] The primary medium is: on the basis of ER medium, add potassium iodate 0.3mg / L, add 2,4-D 0.7mg / L, add paclobutrazol 1.25mg / L;
[0025] The subculture medium is: on the basis of MS medium, first adjust the concentration of ammonium nitrate to 1250mg / L, then add urea 110mg / L, add ABA 3.2mg / L, add indole acetic acid 0.7mg / L, add Glutamine 5.2mg / L, add activated carbon 250mg / L;
[0026] The rooting medium is: in N 6 On the basis of the culture medium, 0.015 mg / L of ammonium molybdate, 1.8 mg / L of TDZ and 140 mg / L of amylopectin were added, and the pH was adjusted to 7.1 with sodium hydroxide.
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