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Method for efficiently preserving Sclernderma guani

A technology of swollen-legged bee and preservation tube, which is applied in the field of insect breeding to achieve the effects of ensuring the survival rate, improving the production control ability, and improving the storage period

Inactive Publication Date: 2017-02-08
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing literature and research have shown that the low-temperature storage limit of adult Scleroderma tubei is about 120 days, and there is no prior art that shows that Scleroderma tube can be stored under refrigerated cocoons

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Place the pupae of the coffee tiger beetle that was parasitized by Scleroderma guanidei larvae into a transparent culture box with a paper pad at the bottom for routine cultivation until the larvae of Scleroderma guanidei cocoon for 8 hours, then take out the cocoons Remove the debris on the pad paper, place it in an incubator with a humidity of 40% and a temperature of 30°C for 6 days, take it out and cool it down to 1°C at a rate of 2°C per hour, and transfer it to a constant temperature refrigerator at 1°C for storage 120 days. After taking out the refrigerated cocoons, raise the temperature to 8°C within one hour and maintain the temperature for 4 hours; then transfer to an environment with light of 200 lux, humidity of 50%, and temperature of 20°C for 72 hours, and then the bee emerges.

Embodiment 2

[0015] Embodiment 2: Place the mature larvae of the corn borer that are parasitized by Scleroderma guanzi to lay eggs and place them in a transparent culture box with pad paper at the bottom for conventional culture until the larvae of Sclerodomina guanzi cocoon for 24 hours, then take out the cocooned larvae. Remove the sundries on the pad paper, place it in an incubator with a humidity of 60% and a temperature of 20°C for 8 days, take it out and cool it down to 4°C at a rate of 4°C per hour, and transfer it to a constant temperature refrigerator at 4°C for 180 sky. After the refrigerated cocoons are taken out, the temperature is raised to 12°C within one hour and maintained at this temperature for 6 hours, and then transferred to an environment with a light of 500 lux, a humidity of 70%, and a temperature of 30°C for 48 hours, and then the bee emerges.

Embodiment 3

[0016] Example 3: Place the mature larvae of the chestnut weevil parasitized by Scleroderma guanidei into a transparent culture box with a paper pad at the bottom for routine cultivation until the larvae of Scleroderma guanidei cocoon for 20 hours, then take out the cocoons Remove the debris on the pad paper, place it in an incubator with a humidity of 40% and a temperature of 20°C for 8 days, take it out and cool it down to 1°C at a rate of 4°C per hour, and transfer it to a constant temperature refrigerator at 1°C for storage 360 days. After the refrigerated cocoons are taken out, the temperature is raised to 8°C within one hour and maintained at this temperature for 6 hours, and then transferred to an environment with light of 200 lux, humidity of 50%, and temperature of 30°C for 48 hours and then the bee emerges.

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PUM

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Abstract

The invention relates to a method for efficiently preserving Sclernderma guani, belonging to the technical field of insect cultivation. The method comprises the following steps: putting mated Sclernderma guani female bees on insect larvae or pupae in the early pupation period to lay eggs; after culture and cocooning, growing in a culture box at 20-30 DEG C under the humidity of 40-60% for 6-8 days; and cooling to 1-4 DEG C at the rate of 2-4 DEG C / hour, and preserving the bee cocoons for 120-360 days. When the Sclernderma guani is in use, the process comprises the following steps: heating the preserved bee cocoons to 8-12 DEG C at the rate of 2-4 DEG C / hour, and keeping the temperature for 4-6 hours; transferring the bee cocoons to natural illumination conditions or an artificial lighting envelopment with the illuminance of 200-500lux, and reviving at 20-30 DEG C under the humidity of 50-70% for 48-72 hours, thereby naturally emerging into Sclernderma guani adults. The cold storage time of the Sclernderma guani can be prolonged to 180-360 days at the low temperature of 1-4 DEG C in the form of cocoons, and the survival percent after long-term freezing can be ensured.

Description

technical field [0001] The invention belongs to the technical field of insect breeding. Background technique [0002] Scleroderma guani Xiao et Wu, belonging to Hymenoptera and Scleroderma family, is a biological control insect that can be widely used to control Lepidoptera, Coleoptera, and Hymenoptera pests. It has considerable agricultural economic potential. For the preservation and use of Scleroderma guanii all the time, the adult bees after eclosion are refrigerated at 3-10°C, and the storage time is ≤120 days. Existing literature and studies have shown that the low-temperature storage limit of adult Scleroderma tubelii is about 120 days, and there is no prior art that shows that Scleroderma tubei can be stored under the condition of refrigerated cocoons. Contents of the invention [0003] The object of the present invention is to provide a method for efficiently preserving Scleroderma guanii, that is, a method for preserving cocoons of Scleroderma guanii, so as to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02A01K67/033
Inventor 吴国星秦小萍吴道慧高熹何霞红李强郝凯强兰明先
Owner YUNNAN AGRICULTURAL UNIVERSITY
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