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Method for evaluating effect of compound on angiogenesis in pathological state

A pathological state and angiogenesis technology, applied in biological testing, material inspection products, measuring devices, etc., can solve problems such as inability to effectively distinguish blood vessels, and achieve stable, reliable, repeatable, and easy-to-operate effects

Active Publication Date: 2017-02-01
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unfortunately, the current angiogenesis models established using zebrafish models are all direct observation of new blood vessels, which are angiogenesis in a physiological state. Strictly speaking, it cannot effectively distinguish between normal angiogenesis and pathological state. Angiogenesis

Method used

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  • Method for evaluating effect of compound on angiogenesis in pathological state
  • Method for evaluating effect of compound on angiogenesis in pathological state
  • Method for evaluating effect of compound on angiogenesis in pathological state

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Embodiment Construction

[0014] A method for evaluating the effect of a compound on angiogenesis in a pathological state, characterized in that it comprises the following steps:

[0015] (1) Transgenic zebrafish embryos with normal development 48 hours after fertilization were transferred into culture wells, and treated with vascular endothelial growth factor to obtain VEGF-treated zebrafish embryos; Treated as normal zebrafish embryos;

[0016] (2) One group of VEGF-treated zebrafish embryos was continuously administered and incubated until 72hpf, as the dosing group, and another group of VEGF-treated zebrafish embryos was added with the same amount of culture water and incubated to 72hpf, as the VEGF group, normal zebrafish embryos were added for culture Water incubation to 72hpf, as a normal control group;

[0017] (3) For zebrafish in the anesthesia group, VEGF group and normal control group, collect fluorescence microscopic images of the inferior intestinal vein (SIVs) area of ​​each zebrafish, ...

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Abstract

The invention relates to a method for evaluating the effect of a compound on angiogenesis in a pathological state. According to the invention, a vascular endothelial growth factor (VEGF) is used for treating young zebra fish in a specific period to simulate a pathological state, then complete subintestinal peripheral vein length is used as an evaluation index, so the evaluation method for the effect of a drug on angiogenesis in the pathological state is successively established. The method has the advantages of simple operation, rapidness, stability, reliability and good repeatability, is more scientific and objective, presents the generation condition of spinous process and improves accuracy of results.

Description

technical field [0001] The invention relates to a method for evaluating the effect of compounds on angiogenesis under pathological conditions, and belongs to the field of drug screening. Background technique [0002] Angiogenesis is regulated by many complementary and complex signaling pathways in the human body, and is related to the occurrence and treatment of many diseases. In addition to affecting the occurrence of tumors, angiogenesis also plays an important role in the occurrence of other diseases, such as retinopathy, rheumatoid arthritis, atherosclerosis, and endometriosis. Blocking angiogenesis in pathological conditions has also become a new approach for this type of disease. [0003] Studies have shown that vascular endothelial growth factor (vascular endothelial growth factor, VEGF) can directly act on vascular endothelial cells, stimulate their mitosis, thereby promoting the growth of new blood vessels, and is the most important angiogenesis factor. VEGF is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5008
Inventor 韩利文刘可春何秋霞张云韩建王荣春孙晨王雪侯海荣彭维兵陈维云陈锡强张轩铭李晓彬张姗姗楚杰王希敏郭敬兰党立
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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