Use of human MIR135a-3p in preparation of preparations for diagnosing and treating prostate cancer

A prostate cancer and application technology, applied in the field of biotechnology, can solve the problems of difficult diagnosis, low diagnostic coincidence rate, high misdiagnosis rate, etc., and achieve the effect of convenient sensitivity, simple operation and high specificity

Inactive Publication Date: 2017-02-01
FUDAN UNIV +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the limitations of digital rectal examination mainly lie in four aspects: (1) When the prostate mass is not large, it is easy to miss the diagnosis; (2) Some patients have no obvious prostate cancer, but it is already at an advanced stage, and it is not easy to cure; (3) This test cannot be used when there is a disease in the rectum; (4) Inexperienced doctors may miss or misdiagnose
Under normal circumstances, the PSA in the blood is not high (not higher than 4ng/ml). When in the state of prostate cancer and other prostate diseases, the increase of PSA has become the most sensitive tumor marker for screening prostate cancer, but it also exists Certain limitations: (1) Blood testing is required, which will cause certain damage to the patient; (2) Elevated PSA is also common with non-prostate cancer diseases, such as prostatitis, prostatic hypertrophy, etc., so

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of human MIR135a-3p in preparation of preparations for diagnosing and treating prostate cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Through the following main steps in this embodiment:

[0028] 1. Extraction of total RNA from tissue samples

[0029] Obtain post-operative tissue samples from patients undergoing radical prostatectomy and patients who have undergone lymphadenectomy at the same time

[0030] The excised tissue sample was used as a control, and the total RNA of the aforementioned tissue sample was extracted in a 1.5 ml centrifuge tube without DNA and RNase pollution;

[0031] The kit for extracting total RNA from tissue samples was purchased from Beijing Kangwei Century Biotechnology Co., Ltd.; the concentration of the extracted total RNA was determined by using a Therm NanoDrop2000c spectrophotometer to measure the ratio of 260 / 280nm ultraviolet wavelength;

[0032] 2. Quantitative detection of microRNA in tissue samples

[0033] 1) Reverse transcribe RNA to obtain single-stranded cDNA

[0034] The reverse transcription system was configured according to Table 1 below, and the config...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the biotechnical field, and concretely relates to a new prostate cancer molecule marker microRNA 135a-3p (MIR135a-3p) and a use thereof in the preparation of preparations for diagnosing and treating the prostate cancer. The use comprises the following steps: screening prostate cancer high-risk populations, diagnosing the prostate cancer, designing prostate cancer drug target, monitoring the prostate cancer treatment condition, and carrying out prognosis detection on the prostate cancer. A method using the molecule marker and a diagnostic kit containing the molecule marker to diagnose the prostate cancer has the characteristics of simplicity in operation, convenience in material drawing, good safety, no wounds, high specificity, high sensitivity, and easiness in massive screening.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a new prostate cancer molecular marker MIR135a-3p and its use in preparing preparations for diagnosing and treating prostate cancer, including the screening of high-risk groups of prostate cancer through the biomolecular marker , the diagnosis of prostate cancer, the design of prostate cancer drug targets, the monitoring of prostate cancer treatment status, the monitoring of prostate cancer drug guidance and the detection of prostate cancer prognosis and other fields. Background technique [0002] Existing technologies have disclosed that liquid biopsy technology (according to the relevant content in the "ASCO Annual Report: 2015 Clinical Oncology Advances") is listed as one of the next decade trends in the field of cancer treatment. Studies have shown that some molecular markers in the blood have been used in the diagnosis of some diseases, such as alanine aminotransferas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/113C12Q1/68
Inventor 李瑶鲍升林景奉香杨树浦洪雷吴海
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap