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Method and reagent kit for detecting expression quantities of TROP2 (trophoblastic cell surface antigen 2) proteins

A technology of protein expression and detection method, applied in the field of detection method and kit of TROP2 protein expression, can solve the problem of undetected expression and prognosis of bladder cancer

Inactive Publication Date: 2017-01-11
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still a lack of a scientific method for detecting the expression level of TROP2 protein so far
Meanwhile, the expression and prognostic role of TROP2 in bladder cancer have not been examined

Method used

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  • Method and reagent kit for detecting expression quantities of TROP2 (trophoblastic cell surface antigen 2) proteins
  • Method and reagent kit for detecting expression quantities of TROP2 (trophoblastic cell surface antigen 2) proteins
  • Method and reagent kit for detecting expression quantities of TROP2 (trophoblastic cell surface antigen 2) proteins

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Embodiment 1

[0036] 1. Materials and methods

[0037] 1. Patient and tissue samples

[0038] For immunohistochemical detection, a total of 102 paraffin-embedded bladder transitional cell carcinomas and 23 normal bladder tissue specimens were collected from ** Hospital from January 2003 to December 2005. Criteria for collection were histopathological diagnosis of transitional cell carcinoma of the bladder, newly diagnosed and untreated, with no history of other neoplasms, and possible follow-up follow-up. Carcinoma in situ was excluded from this study. Clinical samples were used for research only, with prior consent from patients and approval by the National Hospital Ethics Committee.

[0039] The information of the clinical samples is detailed in Table 1. The patients included 67 men and 35 women, aged 41 to 88, with an average age of 66.1 years. All patients underwent transurethral resection, and all patients received intravesical infusion of mitomycin C (MMC) and pirarubicin (THP), o...

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Abstract

The invention relates to the field of biomedicine, in particular to a method and a reagent kit for detecting expression quantities of TROP2 (trophoblastic cell surface antigen 2) proteins. The method includes staining normal tissues and to-be-detected sample tissues by the aid of immunohistochemical technologies and detecting the staining concentration and the staining ranges; acquiring staining concentration fractions according to the staining concentration and acquiring staining range fractions according to the staining ranges; acquiring staining indexes SI according to the staining concentration fractions and the staining range fractions; acquiring the expression quantities of the TROP 2 proteins according to the staining indexes SI. The method and the reagent kit have the advantages that the expression quantities of the TROP2 proteins can be scientifically detected by the aid of the method, high expression of the TROP2 proteins in NMIBC (non-muscle invasive bladder cancer) is discovered, and accordingly the TROP2 proteins are independent prognostic factors for NMIBC.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method and kit for detecting the expression level of TROP2 protein. Background technique [0002] Bladder cancer is one of the most common malignant tumors, with approximately 72,570 new cases and 15,210 deaths from bladder cancer in the United States in 2013. About 80% of bladder cancers at first diagnosis are non-muscle invasive bladder cancer (NMIBC), which can be treated by combining urethrectomy and bladder infusion. However, about 50% to 70% of NMIBC patients will aggravate and relapse within two years after the first diagnosis, and 20% to 30% of them will develop into muscle-invasive bladder cancer (muscle-invasive bladder cancer, abbreviated as MIBC). [0003] Relapse and progression are the main features of NMIBC. So far, the markers that have been established and routinely used to predict tumor recurrence and progression mainly rely on tumor TNM staging and tumor differe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/68G01N33/57484G01N2800/7028
Inventor 王志伟
Owner SUZHOU UNIV
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