A kind of method of ULC determination of flavonoids in Astragalus root extract

A technology of astragalus extract and flavonoids, applied in the field of determination of four flavonoids in astragalus extract, can solve the problem of lack of detection methods for flavonoids

Active Publication Date: 2020-04-03
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For Astragalus extract, a complete set of comprehensive process and quality control system has been established, but there is still a lack of detection methods for the flavonoids in Astragalus extract

Method used

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  • A kind of method of ULC determination of flavonoids in Astragalus root extract
  • A kind of method of ULC determination of flavonoids in Astragalus root extract
  • A kind of method of ULC determination of flavonoids in Astragalus root extract

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] The chromatographic conditions and system suitability test instrument is Waters ACQUITY ultra-high performance liquid chromatography, using UPLCHSS T3 chromatographic column, with 0.2% formic acid aqueous solution as mobile phase A, acetonitrile as mobile phase B, the gradient setting is shown in the table below, and the flow rate is 0.4mL / min; detection wavelength: 254nm.

[0116]

[0117] Preparation of the reference substance solution Take an appropriate amount of calycosin, calycosin glucoside, formononetin and formononetin reference substance respectively, add 70% methanol to prepare each milliliter containing calycosin, calycosin glucoside, formononetin and Formononetin is a solution of 0.01 mg, 0.04 mg, 0.004 mg and 0.15 mg, respectively.

[0118] Preparation of the test solution Take about 0.5 g of Radix Astragali extract, weigh it accurately, add an appropriate amount of 70% methanol solution, dissolve it with ultrasonic waves, dilute to 25 ml, shake well, ...

Embodiment 2

[0124] Chromatographic conditions and system suitability test instrument is Waters ACQUITY ultra-high performance liquid chromatography, using UPLCHSS T3 chromatographic column, with 0.1% formic acid aqueous solution as mobile phase A, 0.1% formic acid acetonitrile as mobile phase B, the gradient settings are shown in Table 1, the flow rate 0.3mL / min; detection wavelength: 254nm.

[0125] Preparation of reference substance solution Take appropriate amount of calycosin, calycosin glucoside, formononetin and formononetin reference substance respectively, add methanol to prepare each milliliter containing calycosin, calycosin glucoside, formononetin and formononetin Anthocyanins are 0.01mg, 0.04mg, 0.004mg and 0.15mg solutions respectively.

[0126] Preparation of the test solution Take about 0.5 g of Radix Astragali extract, weigh it accurately, add an appropriate amount of 50% methanol solution, dissolve it with ultrasonic waves, dilute to 25 ml, shake well, and you get it.

...

Embodiment 3

[0129] Chromatographic conditions and system suitability test equipment is Waters ACQUITY ultra-high performance liquid chromatography, using UPLCHSS T3 chromatographic column, with 0.5% formic acid aqueous solution as mobile phase A, 0.5% formic acid acetonitrile as mobile phase B, the gradient settings are shown in Table 1, the flow rate 0.6mL / min; detection wavelength: 280nm.

[0130] Preparation of reference substance solution Take appropriate amount of calycosin, calycosin glucoside, formononetin and formononetin reference substance respectively, add methanol to prepare each milliliter containing calycosin, calycosin glucoside, formononetin and formononetin Anthocyanins are 0.01mg, 0.04mg, 0.004mg and 0.15mg solutions respectively.

[0131]Preparation of the test solution Take about 0.5 g of Radix Astragali extract, weigh it accurately, add an appropriate amount of 50% methanol solution, dissolve it with ultrasonic waves, dilute to 25 ml, shake well, and you get it.

[0...

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Abstract

The invention relates to a method for simultaneously determining four flavone components in a radix astragali extract by UPLC (ultra performance liquid chromatography). The method comprises the following steps: 1. preparation of reference substance liquid: respectively taking calycosin, calycosin-7-glucoside, onocerin and ononin reference substances, and adding 50-80% methanol to prepare a liquid containing 0.01mg / ml calycosin, 0.04mg / ml calycosin-7-glucoside, 0.004mg / ml onocerin and 0.15mg / ml ononin; 2. preparation of test sample liquid: taking 0.5g of a radix astragali extract, extracting with 50-80% methanol, adding the solvent into the extracting liquid until the volume is 25ml, and shaking uniformly; and 3. determination: respectively absorbing 1-3 mu l of the reference substance liquid and 1-3 mu l of the test sample liquid, injecting into a liquid chromatograph, recording the peak area, and calculating the contents of the calycosin, calycosin-7-glucoside, onocerin and ononin by an external standard process.

Description

technical field [0001] The invention relates to a method for determining traditional Chinese medicine extracts by ultra-high performance liquid chromatography, in particular to a method for determining four flavonoid components in the astragalus extract. Background technique [0002] Astragalus is the root of the leguminous plants Astragalus mongolica and Astragalus capsularis, which can enhance the immune function of the body, protect the liver, diuretic, anti-aging, anti-stress, lower blood pressure and a wider range of antibacterial effects. Astragalus mainly contains three main components, namely triterpenoid saponins, flavonoids and polysaccharides. [0003] Flavonoids (flavonoids) are a class of compounds that exist in nature and have a 2-phenylchromone (flavone) structure. According to the degree of oxidation of the three-carbon bond (C3) structure and the connection position of the B ring, flavonoids can be divided into the following categories: flavones and flavono...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 牛涛徐波王苹陈红孙茜
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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