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Pulmonary fibrosis animal model and preparation method thereof, and application of chrysotile

A pulmonary fibrosis, animal model technology, applied in the field of experimental zoology, can solve the problems of limited pulmonary fibrosis, self-healing, endothelial cell damage, etc., to overcome self-healing tendency, high model success rate, simple operation Effect

Inactive Publication Date: 2017-01-11
TIANJIN HAIHE HOSPITAL
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Problems solved by technology

However, the bleomycin animal model of pulmonary fibrosis also has certain limitations: 1. Part of the bleomycin-induced pulmonary fibrosis can improve on its own and has a tendency to self-heal; 2. The animal model of bleomycin pulmonary fibrosis is endothelial cell Impaired, can not mimic human pulmonary fibrosis triggered by epithelial cell damage; 3 Glucocorticoids can effectively control bleomycin-induced pulmonary fibrosis in animals, but have limited effect on human idiopathic pulmonary fibrosis

Method used

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  • Pulmonary fibrosis animal model and preparation method thereof, and application of chrysotile
  • Pulmonary fibrosis animal model and preparation method thereof, and application of chrysotile

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Embodiment 1

[0020] 1. Modeling:

[0021] (1) Animal grouping: 20 male C57BL / 6 mice aged 6-8 weeks were randomly divided into a model group and a control group, 10 in each group; the model group was a chrysotile fiber group, and the control group was a titanium dioxide group;

[0022] In this project, chrysotile, with a particle size of about 0.5-2.0 μm and fibers with a length of about 0.2-3.0 μm, accounted for more than 90%, was dissolved in sterilized PBS phosphate buffer solution to make a 2 mg / ml stock solution; similarly , titanium dioxide is also made into 2mg / ml PBS stock solution;

[0023] (2) Mice were anesthetized by intraperitoneal injection of 100 μl pentobarbital sodium (10 g / l), and non-exposed tracheal instillation was carried out after the anesthesia took effect. Insert a sterile plastic tube with a 1ml syringe at the end into the trachea of ​​the mouse, and instill the chrysotile solution; after the infusion, stand the mouse upright, and rotate the mouse vertically to ma...

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Abstract

The invention relates to the field of experimental zoology, in particular to a pulmonary fibrosis animal model and a preparation method thereof, and application of chrysotile. Chrysotile fiber is used as an inducer and is instilled to SPF-level C57BL / 6 mouse by non-exposure process via intratracheal instillation, pulmonary fibrosis may be successfully induced in 21 days, operating is simple, model success rate is high, good conformity to pulmonary fibrosis process is achieved, post-forming stability is good, the fibrosis degree is irreversible and worsens gradually, self-healing tendency possible to a bleomycin model is overcome, and the model provides reliable animal model basis for the deep study on molecular mechanisms of pulmonary fibrosis.

Description

technical field [0001] The invention relates to the field of experimental zoology, in particular to a novel pulmonary fibrosis model, a preparation method and the application of chrysotile. Background technique [0002] Pulmonary fibrosis is a progressive and lethal disease, the mechanism of which remains to be elucidated. There is no effective treatment other than lung transplantation. Therefore, establishing a simple and stable animal model consistent with the mechanism of pulmonary fibrosis is of great significance for in-depth research on the mechanism of pulmonary fibrosis and the development of targeted therapeutic drugs. [0003] The animal model of pulmonary fibrosis is an indispensable experimental method for studying the mechanism of pulmonary fibrosis and new drugs. Using animal models, researchers can controllably observe the various stages of disease occurrence and development as well as the pathomorphological and molecular biological manifestations of the dis...

Claims

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Application Information

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IPC IPC(8): A61K33/12A01K67/02
CPCA01K67/02A61K33/12
Inventor 李莘梁玉娥徐龙王勇王咏梅武俊平
Owner TIANJIN HAIHE HOSPITAL
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