Device for fluidization loading or taking out low-temperature protection agent for biological tissue

Abstract

The invention relates to a device for fluidization loading or taking out a low-temperature protection agent for a biological tissue. The device is characterized by comprising a solution circulating module, a solution supplying module, a sample container, a temperature control box and a controller. The sample container is arranged in the temperature control box; the sample container is provided with a liquid inlet and a liquid outlet; the solution circulating module communicates with the liquid inlet and the liquid outlet of the sample container, so that a solution flows through the sample container in a circulating way; the solution supply module communicates with the solution circulating module so as to supply the solution to the solution circulating module; the controller is respectively and electrically connected with the solution supplying module, the solution circulating module and the temperature control box. According to the automation device, the threshold treatment temperature can be reduced, and meanwhile, the speed rate for loading and taking the low-temperature protection agent in and out of the biological tissue is accelerated.

Description

technical field [0001] The invention relates to the technical field of cryopreservation, in particular to a device for fluidized loading or taking out a cryoprotectant of biological tissue. Background technique [0002] Cryopreservation refers to cooling the living biological samples to a low temperature by a special method, usually at -196°C and storing them for a long time; when needed, the biological samples can be heated to normal temperature by a special method, and the living biological samples can still be obtained. When cryopreserving biological samples, except for very simple microorganisms, viruses and bacteria, it is generally necessary to add cryoprotectants. [0003] Although cryoprotectants can protect biological samples at low temperatures, enough cryoprotectants must be loaded into biological samples in order for this protection to work. However, cryoprotectants are foreign substances to biological samples and are somewhat toxic to them. This toxicity is cl...

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Problems solved by technology

[0004] Therefore, if the lowest limit treatment temperature can be lowered below the equilibrium freezing point temperature, that is, supercooling can be enhanced, and at the same time, the rate of penetration of cryoprotective agents into biological tissues can be accelerated, the toxic damage suffered by biological tissues can be greatly reduced, that is, the The success rate of cryopreservation of biological tissues has been greatly improved, but there is no automated device that can achieve this function

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