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Method for cultivating large yellow croaker fingerling and screening molecular marker applied to method

A molecular marker, a technology for large yellow croaker, applied in biochemical equipment and methods, applications, fish farming, etc., can solve the problems of rare genetic markers, weak application research, etc., to improve the utilization rate of feed and the stress of broodstock Small, low-cost effect

Active Publication Date: 2016-12-07
宁德市富发水产有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0006] At present, there are few reports on the analysis of genetic markers related to the economic traits of large yellow croaker, especially the genetic selection at the molecular level is still in the theoretical research stage, and the application research in production practice is still relatively weak

Method used

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  • Method for cultivating large yellow croaker fingerling and screening molecular marker applied to method
  • Method for cultivating large yellow croaker fingerling and screening molecular marker applied to method
  • Method for cultivating large yellow croaker fingerling and screening molecular marker applied to method

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0083] Experimental animals:

[0084] The large yellow croaker in this experiment was self-bred by Ningde Fufa Aquatic Products Co., Ltd.

[0085] Experimental reagents:

[0086] Generay company cell / tissue genomic DNA extraction kit: spin column type GK0222, produced by Generay company;

[0087] DNA marker kit: GsDL2501-100, produced by Shanghai Jierui Bioengineering Co., Ltd.;

[0088] Taq enzyme: produced by Shanghai Jierui Bioengineering Co., Ltd.

[0089] The following is attached with the manual Figure 1-10 The technical solution of the present invention is described in detail.

[0090] A kind of method of cultivating large yellow croaker fry of the present invention comprises following steps carried out in sequence:

[0091] 1) Screen the growth axis-related genes of large yellow croaker and their linked SSRs: According to the reported functions of growth-related genes of other species and the published genetic information of the whole genome of large yellow croaker...

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Abstract

The invention relates to a method for cultivating large yellow croaker fingerling and a screening molecular marker applied to the method. The method comprises the following steps: 1) screening the related genes of growth axis of the large yellow croaker and the chain SSR thereof; 2) compounding a primer; 3) detecting SSR primer extension polymorphism; 4) marking and screening the growth related SSR; 5) marking and screening parent fish of large yellow croaker; 6) cultivating the large yellow croaker fingerling. The method can overcome the defects being short of rapid screening and cultivation for the large yellow croaker fingerling of the prior art and has the advantages of high speed, convenience, effectiveness and capability of accelerating the cultivating process of large yellow croaker.

Description

technical field [0001] The invention relates to a method for cultivating large yellow croaker fry and a screening molecular marker used therein, which are applied to cultivating high-quality large yellow croaker varieties with fast production. Background technique [0002] Large yellow croaker (Larimichthys crocea) is a unique seawater economic fish in my country. It is known as the "national fish". And 33.15% and 10.75% of the aquaculture output, with an annual output value of more than 10 billion yuan. It is the largest marine aquaculture fish in my country and one of the eight advantageous export aquaculture aquatic products. However, due to the lack of scientific and effective management measures in the long-term extensive breeding process, the germplasm of large yellow croaker is facing many problems such as slow growth, premature sexual maturity, individual miniaturization and decline in disease resistance, and germplasm improvement is urgently needed , in order to bre...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11A01K61/00
CPCY02A40/81C12Q1/6888A01K61/00C12Q2600/124C12Q2600/156
Inventor 韩坤煌王艺磊涂传灯饶琳刘卫刚刘家富
Owner 宁德市富发水产有限公司
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