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Agent for regulating composition ratio of intestinal bacterial flora

A technology of intestinal bacteria and ratio, which is applied to plant/algae/fungus/moss ingredients, food ingredients, organic active ingredients, etc., can solve the problems of undiscovered proliferating Bacteroides bacteria, burden, reduction, etc., and improve the immune index , no side effects, and the effect of alleviating allergic symptoms

Inactive Publication Date: 2016-12-07
FUJIFILM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, in order to achieve the effect of ingesting fructo-oligosaccharides, a large amount of intake is required, and ingesting it through diet or functional foods places a burden on the body
In addition, oral substances that have the effect of proliferating bacteria belonging to the phylum Bacteroidetes and also reducing bacteria belonging to the phylum Firmicutes have not been found

Method used

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  • Agent for regulating composition ratio of intestinal bacterial flora
  • Agent for regulating composition ratio of intestinal bacterial flora

Examples

Experimental program
Comparison scheme
Effect test

test approach 1

[0036] Sucrase IC 50 Determination of value

[0037] Preparation of sample solution: 2 mg of sample (substance that inhibits α-glucosidase activity) was weighed into a test tube, and 2 mL of water was added thereto, and then the sample was completely suspended to form a sample solution having a concentration of 1 mg / mL. It was diluted with water to form solutions at 0, 50, 100, 250 and 500 μg / mL, respectively.

[0038] Preparation of substrate solution: Sucrose was dissolved in 0.2M maleate buffer (pH 6.0) so that the sucrose concentration was 100 mM, and the solution was used as a substrate solution.

[0039] Preparation of crude enzyme solution: After suspending 1 g of rat intestinal acetone powder rat (manufactured by SIGMA) in 10 mL of physiological saline, the suspension was centrifuged (3000 rpm, 4° C., 5 min). The resulting supernatant was separated and used as crude enzyme solution.

[0040] To 500 μL of the above sample solutions having respective concentrations wa...

Embodiment

[0100] The present invention will be described below with reference to examples, but the present invention is not limited to the following examples.

[0101] After pulverizing the root and stem parts of Salacia reticulata and Salacia oblongatus, they were mixed in equal weight, and the liquid obtained through the hot water extraction step at 98°C was spray-dried to obtain Salacia extract powder1. Salacia extract powder 1 has a sucrase IC50 value of 41 (μg / ml).

[0102] In addition, for Gymnema and mulberry leaf extracts, those available from K.K. Cross can be used.

[0103] Also, for the comparative example, glucose, lard, and oolong tea polymerized polyphenols (obtained by freeze-drying black oolong tea produced by Suntory Ltd. and extracting oolong tea polymerized polyphenols) were used. In addition, for Gymnema, mulberry leaf extract, glucose, lard, and oolong tea polyphenols, their sucrase 50% inhibitory concentrations (IC 50 value) of 800 μg / mL or greater.

[0104] Th...

experiment example 1

[0106] Eight-week-old male SD rats were divided into seven rat groups. Each of the following intestinal bacterial flora composition ratio regulators was dissolved in water for injection, and ingested over a week in an amount equivalent to 20 mg / kg / day using a gastric feeding tube (stomach sonde). The proportion of Bacteroides and Firmicutes relative to the gut bacterial community was determined by the T-RFLP method (Nagashima method) immediately before and immediately after ingestion, respectively. However, in Example 1 half the amount (10 mg / kg / day) of intestinal bacterial flora constituted the ratio regulator was used.

[0107] Table 1 shows the proportions of Bacteroides and Firmicutes relative to the intestinal bacterial community (proportions obtained when the total number of bacteria is taken as 100%).

[0108]

[0109] Regarding the results of this experimental example, it shows that the sugar absorption inhibiting substance significantly increases the ratio of Bact...

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Abstract

A food or pharmaceutical capable of regulating an intestinal bacterial flora distribution ratio, which is safe and has few side effects, is provided. An intestinal bacterial flora distribution ratio regulator containing at least one sugar absorption-suppressing substance, and a food or pharmaceutical containing the same.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of April 21, 2010 and an application number of 201080017939.9. technical field [0002] The present invention relates to an intestinal bacterial flora composition ratio regulator comprising a substance that inhibits sugar absorption. Background technique [0003] According to recent studies, it has been found that in humans, obese people are in a state in which the proportion of Bacteroidetes bacteria is low and the proportion of Firmicutes bacteria is high, and the proportion of Bacteroidetes increases with weight loss , and on the contrary, the proportion of Firmicutes decreased. Furthermore, it has been found that lean persons have a high composition ratio of Bacteroidetes and a low composition ratio of Firmicutes relative to obese persons (see Non-Patent Document 1). [0004] Furthermore, when the gut bacterial flora of obese mice and that of normal-weight mice w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/37A61P3/04A23L33/105
CPCA61K36/37A61K2236/331A23V2002/00A61K2300/00A23V2200/332A23V2250/21A61K31/00A61K36/27A61K36/605A23L33/105A61P1/00A61P1/14A61P11/02A61P3/04A61P35/00A61P37/04A61P37/08A61P43/00A23V2200/30
Inventor 小田由里子植田文教
Owner FUJIFILM CORP
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