Use of biological marker detection reagent in preparing kit for detecting invasiveness of pituitary adenomas

A technology of biomarkers and detection reagents, applied in the field of biomedicine, can solve problems such as difficult to achieve total resection

Active Publication Date: 2016-11-23
BEIJING NEUROSURGICAL INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Can infiltrate and grow into surrounding structures such as sphenoid sinus, cavernous sinus on both

Method used

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  • Use of biological marker detection reagent in preparing kit for detecting invasiveness of pituitary adenomas
  • Use of biological marker detection reagent in preparing kit for detecting invasiveness of pituitary adenomas
  • Use of biological marker detection reagent in preparing kit for detecting invasiveness of pituitary adenomas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The clinical data of 312 cases of pituitary adenomas were collected and divided into 135 cases in the invasion group and 177 cases in the non-invasive group according to the Knosp classification. Construct a tissue chip to detect the expression levels of EGFL7, Notch3, EGFR, β-catenin and c-Myc protein in pituitary adenoma specimens using the Leica-BOND III system to perform fully automated immunohistochemical methods, and analyze the above-mentioned proteins and pituitary adenoma invasion, etc. Correlation of clinicopathological characteristics.

[0040] Use Leica automatic scanner to scan, analyze after scanning, give high and low expression interpretation. When the proportion of EGFL7, Notch3, EGFR, c-Myc, β-catenin expressing color 2 points and 3 points respectively reaches 51%-100%, it is determined as high expression, and low expression is the proportion of cells expressing color 1 point 1-100% or 2 points and 3 points are less than 50% of the cells.

[0041] The res...

Embodiment 2

[0046] The method of Example 1 was used to detect the expression level of biomarkers in the 312 pituitary adenoma specimens (including 135 invasive specimens and 177 non-invasive specimens) in Example 1. The only difference is that each specimen was performed 6 times Parallel detection, the types of biomarkers detected each time are shown in Table 2.

[0047] Table 2

[0048]

[0049] In the first group, specimens with high expression of EGFL7, Notch3, EGFR, and c-My were identified as the invasion group, and 74 cases of invasion were detected.

[0050] In the second group, the specimens with high expression of EGFL7, Notch3, EGFR and β-catenin were identified as invasive specimens, and a total of 99 invasive specimens were detected.

[0051] In the third group, the specimens with high expression of EGFL7, Notch3, c-My and β-catenin were identified as invasive specimens, and 77 invasive specimens were detected.

[0052] In the fourth group, the specimens with high expression of EGFL7, ...

Embodiment 3

[0057] In this example, the Transwell experiment was used to identify the invasion ability of pituitary adenoma cells.

[0058] 1. Melt BD Matrigel as a liquid reserve.

[0059] 2. Dilute Matrigel with serum-free medium at a volume ratio of 1:8 (Matrigel: serum-free medium).

[0060] 3. Add 80μL of the diluted Matrigel working solution to the upper chamber of the Transwell chamber, and incubate it in an incubator at 37°C for 6 hours to polymerize the Matrigel gel.

[0061] 4. When the pituitary adenoma cells (GH3, purchased from ATCC) grow to a confluence rate of 85%, when they are in good condition, digest the cells, resuspend the cells in serum-free medium, and count and adjust the density to 2×10 5 Cells / ml to obtain cell suspension.

[0062] 5. Including five treatment groups, among which treatment groups 1-3: add about 200μl of the above cell suspension in the upper chamber of the Transwell chamber (24-well plate, 8-plate), and divide it into 3 different treatment groups to add the...

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Abstract

The invention discloses a use of a biological marker detection reagent in preparing a kit for detecting the invasiveness of pituitary adenomas, and a kit and a biological marker combination for detecting the invasiveness of pituitary adenomas. The invasiveness of pituitary adenomas of a patient can be detected by detecting an expression level of a biological marker, so that the grade malignancy of the pituitary adenomas can be known, and an effective basis can be provided for knowing the prognosis of the pituitary adenomas and instructing the clinical diagnosis and treatment.

Description

Technical field [0001] The invention relates to the field of biomedicine, in particular to the use of a biomarker detection reagent in preparing a kit for detecting the invasiveness of pituitary adenomas. Background technique [0002] Pituitary adenomas account for 10%-15% of primary intracranial tumors and are one of the most common neurological tumors. Although pituitary adenomas are benign tumors as a whole, some tumors are between benign and malignant, and tumors grow aggressively. Jefferson first proposed the concept of aggressive pituitary adenoma in 1940. Martins in 1965 defined it as: "pituitary adenoma growth breaks through the capsule or invades adjacent structures". It is generally believed that invasive pituitary adenoma is a pathological type between benign adenoma and pituitary adenocarcinoma. Its histological morphology is benign but its biological characteristics are similar to malignant tumors. It can infiltrate and grow into the sphenoid sinus, cavernous sinu...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57407
Inventor 刘潜高华张亚卓刘春晖姚晓辉李储忠
Owner BEIJING NEUROSURGICAL INST
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