Method for improving yield of erythromycin through saccharopolyspora erythraea SACE_Lrp gene

Abstract

The invention discloses a method for improving the yield of erythromycin through a saccharopolyspora erythraea SACE_Lrp gene. The SACE_Lrp gene is inactivated in saccharopolyspora erythraea through a genetic engineering way, and a saccharopolyspora erythraea erythromycin high-yield engineering strain is obtained through excessively expressing a target gene SACE_5387-5386; the erythromycin is produced through fermenting the obtained strain, and the strain obtained by the technology is fermented, so that the yield of the erythromycin can be improved.

Description

technical field [0001] The present invention mainly relates to a method for increasing the yield of erythromycin by fermentation, in particular to a method for increasing the yield of erythromycin by inactivating the SACE_Lrp gene and / or overexpressing the SACE_Lrp target gene in Saccharopolyspora erythromycetes. Background technique [0002] Secondary metabolites of actinomycetes have a wide range of uses, such as antibiotics, anticancer agents, immunomodulators, insect repellents, and insect control agents. Among the 23,000 biologically active secondary metabolites discovered so far, more than 10,000 are produced by actinomycetes. However, the original production of these secondary metabolites is very low, and screening is required to obtain high-yielding strains for industrial production. In the past, industrial production strains were mainly obtained by random physical or chemical mutagenesis. The traditional random mutagenesis technique is not only time-consuming, but...

AI Technical Summary

Problems solved by technology

In 2007, Oliynyk et al. reported the genome sequence of Saccharopolyspora NRRL23338, but there are few studies on the regulatory genes of Saccharopolyspora. So far, only the regulatory genes of bldD (SACE_2077), SACE_7040, SACE_0012 and SACE_5599 have been reported.

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