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Culture system for static sterilization curve for anti-anaerobic agent and construction method of culture system

A culture system and anaerobic bacteria technology, applied in the field of in vitro pharmacodynamics of antibacterial drugs, can solve the problems of inconvenient operation and difficulty in anaerobic bacteria research, and achieve the effect of easy and cheap operation

Inactive Publication Date: 2016-11-23
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the special living habits of anaerobic bacteria, it is difficult to study the in vitro sterilization curve of anaerobic bacteria; currently, the research on the sterilization curve of anaerobic bacteria requires special equipment such as anaerobic boxes (with built-in shakers), and its operation has great inconvenience

Method used

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  • Culture system for static sterilization curve for anti-anaerobic agent and construction method of culture system
  • Culture system for static sterilization curve for anti-anaerobic agent and construction method of culture system
  • Culture system for static sterilization curve for anti-anaerobic agent and construction method of culture system

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Effect test

Embodiment 1

[0034] like figure 1As shown, the culture system used for the static sterilization curve of anti-anaerobic bacteria drugs is mainly composed of anaerobic bag 1, culture bottle 2, agitator 3, peristaltic pump 5 and pipeline 7; the anaerobic bag 1 and culture bottle 2 Connected by a pipeline 7, the pipeline 7 is provided with a peristaltic pump 5, the culture bottle 2 is connected to the stirrer 3, the culture bottle 2 is provided with a sampling hole 6, and the inside of the culture bottle 2 is placed stirring bar 4;

[0035] The anaerobic bag 1 can be in the form of one or more series; the anaerobic bag 1 is in the form of a pouch in shape and has an opening for filling anaerobic agent, which can be sealed by clamping, and its shape can be arbitrary shape; the anaerobic bag 1 is provided with at least two interfaces docked with the pipeline 7 (the interfaces on the bag can be any number in the form of combining by shunting); the anaerobic bag 1 usually adopts a soft The anae...

Embodiment 2

[0043] like figure 2 As shown, the cultivation system for the static sterilization curve of anti-anaerobic bacteria medicine of the present invention mainly is made up of anaerobic bag 1, culture bottle 2, agitator 3, peristaltic pump 5 and pipeline 7; Described anaerobic bag 1 and cultivation The bottle 2 is connected by a pipeline 7, the pipeline 7 is provided with a peristaltic pump 5, the culture bottle 2 is connected with the stirrer 3, the culture bottle 2 is provided with a sampling hole 6, and the culture bottle 2 is placed inside There is a stirring bar 4;

[0044] The anaerobic bag 1 can be in the form of one or more series; the anaerobic bag 1 is in the form of a pouch in shape and has an opening for filling anaerobic agent, which can be sealed by clamping, and its shape can be arbitrary shape; the anaerobic bag 1 is provided with at least two interfaces docked with the pipeline 7 (the interfaces on the bag can be any number in the form of combining by shunting); ...

Embodiment 3

[0051] To study the bactericidal effect of left-ornidazole on Bacteroides fragilis, it is necessary to establish a growth model of Bacteroides fragilis, and adopt the culture system and method described in the present invention to establish the growth model; the selected bacteria are the standard strain of Bacteroides fragilis (ATCC 25285) , the initial bacterial concentration was set to 1×10 6 CFU / ml.

[0052] Configure 30ml Brucella broth in the culture bottle, add a stirring bar in each, connect the culture bottle and the pipeline to assemble, and carry out overall disinfection; after disinfection, connect the anaerobic bag to form a closed loop, and put one of the tubes Embed a peristaltic pump in the road, and turn on the peristaltic pump for gas circulation. The speed of the peristaltic pump is set to 5ml / min; the culture bottle part of the system is preheated at 37°C; Put the suspended bacterial solution into the culture bottle, turn on the agitator to stir and cultiv...

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Abstract

The invention belongs to the technical field of in-vitro pharmacodynamics of antibacterial agents and relates to a culture system for a static sterilization curve for an anti-anaerobic agent and a construction method of the culture system. The culture system mainly comprises one or more anaerobic bags, one or more culture bottles, one or more stirrers, a peristaltic pump and pipelines, wherein the one or more anaerobic bags are connected with the one or more culture bottles through the pipelines; the peristaltic pump is arranged on one of the pipelines; the one or more culture bottles are connected with the one or more stirrers; one or more sampling holes are formed in the one or more culture bottles; a stirring bar of each stirrer penetrates through a bottom plate of the corresponding culture bottle and is arranged in the corresponding culture bottle. Use results indicate that the culture system has the advantages being simple, cheap, easy to operate and the like and can be used to, but not limited to, research on the static sterilization curve for the anti-anaerobic agent.

Description

technical field [0001] The invention belongs to the technical field of in vitro pharmacodynamics of antibacterial drugs, and relates to a cultivation system for research on the efficacy of anti-anaerobic bacteria drugs, in particular to a culture system and a construction method for static sterilization curves of anti-anaerobic bacteria drugs. Background technique [0002] The prior art discloses that anaerobic bacteria are a type of bacteria that can only grow in anaerobic or hypoxic environments, and their energy metabolism mainly depends on anaerobic glycolysis; it is reported that common anaerobic bacteria that cause human infection include: Bacteroides fragilis, Clostridium perfringens, Clostridium difficile, etc. In current clinical treatment, the main anti-anaerobe drugs are nitroimidazoles (such as metronidazole, tinidazole, ornidazole, etc.). [0003] In addition, it is currently known that the static bactericidal curve is based on adding a specific concentration o...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/24C12M1/04C12M1/02C12M1/00
Inventor 梁旺胡佳丽赵苗陈渊成张菁徐少华吴湜黄海辉
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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