Efficient breeding method of triploid pure line of Trionychidae

A triploid, pure line technology, applied in animal husbandry and other directions, can solve the problems of high-efficiency breeding of tetraploid all-male soft-shelled turtles, etc., and achieve the effects of improving the breeding rate and emergence rate, inhibiting DNA splitting and reducing trouble.

Inactive Publication Date: 2016-11-23
HUNAN UNIV OF ARTS & SCI
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Problems solved by technology

But in the genetic breeding of soft-shelled turtles, so far, not only have there been no reports on the high-efficiency breeding of tetraploid all-male soft-shelled turtles, but there has also been no precedent for backcrossing all-male tetraploids with diploid female parents.

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  • Efficient breeding method of triploid pure line of Trionychidae

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Embodiment 1

[0015] The high-efficiency breeding method of triploid pure line of soft-shelled turtle comprises the following steps:

[0016] (1) Provision of tamoxifen feeding material: Take tamoxifen powder with a purity of more than 95%, add it to the conventional feeding feed of soft-shelled turtles, and make a feeding material, in which the content of tamoxifen is 10g / 500g feed ,stand-by;

[0017] (2) Feeding: Before the female turtle lays eggs, feed tamoxifen feeding material to the female parent according to the conventional feed feeding method, three times a day, 15g feeding material per 100g turtle at 8:00 in the morning, and 15g feeding material per 100g turtle at noon. At 12:00, every 100g of soft-shelled turtle was fed with 15g of feeding material, at 6:00 pm, every 100g of soft-shelled turtle was fed with 10g of feeding material until laying eggs;

[0018] (3) Cold shock treatment: In the first cleavage, adopt the method of cold shock, put the fertilized eggs in step (2) into ...

Embodiment 2

[0023] The high-efficiency breeding method of triploid pure line of soft-shelled turtle comprises the following steps:

[0024] (1) Preparation of tamoxifen feeding material: Take tamoxifen powder with a purity of more than 95% and add it to the conventional feeding feed for soft-shelled turtles to make a feeding material, in which the tamoxifen content is 8g / 500g feed ,stand-by;

[0025] (2) Feeding: Before the female turtle lays eggs, feed the female parent with tamoxifen according to the conventional feeding method, three times a day, 18g per 100g turtle at 8:00 in the morning, and 12:00 at noon. 00 Feed 15g of feeding material per 100g of soft-shelled turtle, and 10g of per 100g of soft-shelled turtle at 6:00 pm;

[0026] (3) Cold shock treatment: In the first cleavage, adopt the method of cold shock, put the fertilized eggs in step (2) into a cold water tank with a temperature of 0°C for 3 minutes, and take them out for use;

[0027] (4) Preparation of estrogen inhibito...

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Abstract

The invention discloses an efficient breeding method of a triploid pure line of Trionychidae, comprising the steps of preparing tamoxifen feed, feeding females of Trionychidae with the tamoxifen feed, cold-shocking, preparing an estrogen inhibitor solution, spraying the estrogen inhibitor solution evenly, and back-crossing to obtain a triploid. Through the method, it is possible to acquire a 100% triploid pure line, breeding rate and emergence rate of triploids are increased, breeding and selection will counter fewer troubles, and more importantly, no pollution is caused to natural aquatic quality and ecological environment.

Description

technical field [0001] The invention relates to a polyploid breeding method for soft-shelled turtles, in particular to a method for efficiently breeding triploid pure lines of soft-shelled turtles. Background technique [0002] Soft-shelled turtles, with Chinese soft-shelled turtles as the best, are also called turtles and soft-shelled turtles. Belonging to Reptiles, Turtles, and Turtles, diploids are common. Turtle meat is delicious and nutritious. It has the functions of clearing away heat and nourishing yin, calming the liver and quenching wind, softening and resolving hard masses, etc. Diploids grow slowly, and the breeding cycle Up to 4-5 years. Due to the sterility characteristics of triploids, soft-shelled turtles show more significant advantages in terms of growth speed, disease resistance, and meat quality. In the prior art, the fastest and most effective way to obtain triploid sterile soft-shelled turtles is to obtain tetraploid soft-shelled soft-shelled soft-she...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 杨品红邹万生韩庆
Owner HUNAN UNIV OF ARTS & SCI
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