Diagnostic kit for fast determining aphis gossypii glover sensitivity to acetamiprid and use method thereof
A diagnostic kit and rapid determination technology, applied in biological testing, measuring devices, material inspection products, etc., to achieve the effects of avoiding increased costs, accurate and reliable results, and shortening diagnostic time
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Embodiment 1
[0024] Such as figure 1 As shown, the diagnostic kit for rapidly measuring the sensitivity of aphids to acetamiprid of the present invention includes a finger tube 1, a drug film 2 and a filler, and the finger tube 1 is a glass tube with one end closed, and the The drug film 2 is an acetamiprid drug film evenly attached to the inner surface of the finger tube 1 , and the filler is detachably filled in the open end of the finger tube 1 . The finger tube 1 is a cylindrical tube, the length of the finger tube 1 is 8-10 cm, and the inner diameter is 2.5-3 cm. The drug film 2 is uniformly applied to the inner wall of the finger tube 1 by rolling acetamiprid acetone solution, and the content of acetamiprid in the drug film 2 is 0.078 μg / cm 2 .
[0025] Preferably, the filler is a tampon, the tampon is a sterilized absorbent cotton ball, and the size of the tampon matches the size of the mouth of the finger tube 1 .
[0026] The method for using the diagnostic kit for quickly dete...
Embodiment 2
[0045] Dissolve acetamiprid original drug with acetone, dilute it into 7.20mg / L acetamiprid acetone solution, take 1ml of the drug solution and add it into a finger-shaped tube with a length of 9cm and an inner diameter of 3cm, and roll it fully so that the drug solution is evenly attached to the A drug film is formed on the inner wall of the finger-shaped tube. After the acetone in the tube is completely volatilized, a cotton plug is plugged into the tube mouth, and the rapid diagnostic kit is completed.
[0046] The prepared diagnostic kits were taken to cucumber fields in Malianwa, Beijing, and Langfang, Hebei, where the melon aphids occurred seriously. In the fields, the leaves with a large number of aphids were picked, and the aphids were gently swept into the diagnostic kits with a small brush. 30 heads per tube, 3 tubes for each population, and the mortality of the melon aphid was checked after 3 hours. The diagnostic results were: the mortality rate of the two populati...
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