Preparation Method And Analytic Method For The Extract Of Antrodia Cinnamomea

Abstract

An optimal preparation and analytic method for the extraction of Antrodia cinnamomea is disclosed. The important parameters for the extraction efficiency of the extract are analyzed using mathematic and statistical experimental designs, and the extract of A. cinnamomea and its particular triterpenoid compounds are quantified and identified using the quantitative NMR and HPLC-tandem MS. Whether the ergostane and lanostane triterpenoid compounds are contained in one drug, health food or other products and the total amounts and individual amounts therein can be analyzed, determined or quantified using the techniques disclosed herein.

Description

technical field [0001] This case is about a preparation and analysis method of Antrodia camphorata extract, especially, this case is about the optimization of the preparation and analysis of an Antrodia camphorata extract, and the ergosterane triterpenoids and / or lanosterane triterpenoids in the Antrodia camphorata extract. An analytical method for the accurate quantification of terpenoids. Background technique [0002] Antrodia cinnamomea (Androdia cinnamomea), also known as camphor mushroom, cinnamomum cinnamomea, etc., is a unique fungal species in Taiwan. It grows on the inner wall of the decayed heartwood of the unique Cinnamomum kanehirai trunk at an altitude of 400 to 2,000 meters, or dies. The dark, damp surface of lodging cinnamon wood. Therefore, it is not easy to find wild fruiting bodies of Antrodia camphorata or to confirm the appearance of this fungal strain of the order Aphyllophorales, and the price of Antrodia camphorata remains high due to the potential me...

AI Technical Summary

Problems solved by technology

However, this method is easy to cause false positives. If other components in the sample to be tested have carboxyl groups, they will also react with specific color reagents and cause misjudgment.

The above two methods are commonly used to detect the total triterpenoids of Antrodia camphorata, but their accuracy is not good, and there is still room for improvement as a quantitative standard

In addition, China Taiwan Patent Publication No. TW 201416673 A discloses a quantitative method for the triterpenoids of Antrodia Cinnamomea, which obtains the fingerprints of Antrodia Cinnamomea by chromatography, and then calculates the ratio of the integrated area of ​​the absorption peak in the specified interval to the external standard, and further calculates the three terpenoid content, but the invention publication did not disclose the sample extraction method and the total content and individual content of ergostane and lanosterane triterpenoids in the sample

In addition, Chinese Taiwan Patent No. I407100 discloses a method for analyzing the triterpenoids of Antrodia camphorata by using high performance liquid chromatography, but does not disclose the method for quantifying the triterpenoids contained in the fruiting body of Antrodia camphorata

Images