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Preparation method of wide-spectrum tumor urinalysis reagent

A tumor and reagent technology, applied in the field of preparation of broad-spectrum tumor urine test reagents, can solve the problems of loss of reagents, increased trouble, insecurity, etc.

Inactive Publication Date: 2016-11-09
江西格朗生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, in the reaction system, the ammonia in the urine makes the mercurous nitrate (mercury ion) black, which will lead to the adverse consequences of mistakenly thinking that there is bile pigment interference
Secondly, Mirren's reagent cannot be directly applied to urine detection, because urine contains a large amount of inorganic salts, which can precipitate with mercury ions and lose the effect of the reagent. Therefore, mercury sulfate and sulfuric acid are added to their reagents as solvents to provide mercury ions. Precipitate those inorganic salt ions
Adding mercuric sulfate and sulfuric acid will increase the trouble of reagent preparation. Moreover, sulfuric acid is highly corrosive, and it is not safe in the preparation work and in the use of reagents. It should be avoided. effect

Method used

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  • Preparation method of wide-spectrum tumor urinalysis reagent
  • Preparation method of wide-spectrum tumor urinalysis reagent

Examples

Experimental program
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Embodiment 1

[0025] A kind of broad-spectrum tumor urine test reagent is made up of two kinds of solutions, by volume ratio, namely A solution: B solution=1:1.5, A solution preparation: take by weighing 64g mercury, be dissolved in the nitric acid 96ml of specific gravity 1.42g / ml, wait for The mercury is completely dissolved, and the volume Va of the mixed solution is 83.3ml; B solution preparation: the concentration of nickel nitrate solution is 0.1g / ml, weigh 20g of nickel nitrate 6 water, dissolve it in an appropriate amount of distilled water, and then add distilled water to constant volume To 125ml, that is to prepare a nickel nitrate solution with a concentration of 0.1g / ml; then, mix the above two solutions evenly to prepare a 208.3ml reagent.

Embodiment 2

[0027] A kind of broad-spectrum tumor urine test reagent is made up of two kinds of solutions, by volume ratio, namely A solution: B solution=1:1.5, A solution preparation: take by weighing 64g mercury, be dissolved in the nitric acid 96ml of specific gravity 1.42g / ml, wait for The mercury is completely dissolved, and the volume Va of the mixed solution is 83.3ml; B solution preparation: the concentration of the nickel nitrate solution is 0.15g / ml, weighs 29.9g of nickel nitrate 6 water, dissolves it in an appropriate amount of distilled water, and then adds distilled water to set When the volume reaches 125ml, it is prepared into a nickel nitrate solution with a concentration of 0.15g / ml; then, the above two solutions are mixed evenly to prepare a 208.3ml reagent.

Embodiment 3

[0029] A kind of broad-spectrum tumor urine test reagent is made up of two kinds of solutions, by volume ratio, namely A solution: B solution=1:1.8, A solution preparation: take by weighing 370g mercury, be dissolved in the nitric acid 643ml of specific gravity 1.42g / ml, wait for The mercury is completely dissolved, and the volume Va of the mixed solution is measured to be 555.5ml; B solution preparation: the concentration of nickel nitrate solution is 0.10g / ml, takes 159.4g of nickel nitrate 6 water, dissolves it in an appropriate amount of distilled water, and then adds distilled water to set When the volume reaches 1000ml, it is prepared into a nickel nitrate solution with a concentration of 0.10g / ml; then, the above two solutions are mixed evenly to prepare a 1555.5ml reagent.

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Abstract

The invention discloses a preparation method of a wide-spectrum tumor urinalysis reagent. The preparation method comprises solution A preparation: making 1 part by weight of metal mercury and 2.1-2.5 parts by weight of nitric acid with a concentration of 1.42g / ml react to produce a mixed solution and measuring a volume Va of the mixed solution, solution B preparation: weighing analytically pure nickel nitrate, dissolving the nickel nitrate in distilled water and adding distilled water into the solution until a concentration of the nickel nitrate solution is in a range of 0.10-0.15g / ml, and mixing: uniformly mixing the solution A and solution B according to a ratio of Va: Vb of 1: 1.5-1.8 to obtain the wide-spectrum tumor urinalysis reagent. The wide-spectrum tumor urinalysis reagent mainly contains mercury nitrate, mercury nitrite, nickel nitrate, nitric acid and a small amount of nitrous acid. The detected object is a phenolic hydroxyl group-containing amino acid derivative in urine. The wide-spectrum tumor urinalysis reagent obtained by the preparation method has high detection accuracy, a low false positive rate and good use safety.

Description

technical field [0001] The invention relates to a preparation method of a broad-spectrum tumor urine test reagent. Background technique [0002] The natural growth of tumors is artificially divided into two stages: the preclinical stage and the clinical stage. Much is known about tumors in the clinical stages, but tumors at this stage are very difficult to treat. Tumors in the preclinical stage quietly undergo a series of molecular changes, and the human body has no specific sensory changes. If tumors in the preclinical stage can be found, and people can be informed to improve their lifestyle, diet and nutrition structure and change the environment in a timely manner, there is a great possibility of self-healing. [0003] Urine detection reagents for amino acid derivatives containing phenolic hydroxyl groups have been developed since the 1980s. From the perspective of application, so far, no one has gone deep into the application research of preclinical tumors; from the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/82
CPCG01N21/82
Inventor 蔡林卫魏谦杨志平
Owner 江西格朗生物技术有限公司
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