Tissue culture medium and culture method of malus spectabilis

Abstract

The invention discloses a tissue culture medium and culture method of malus spectabilis. The tissue culture medium comprises an MS culture medium, 1.0 g / L 6-benzylamino adenine and 0.1 g / L alpha-naphthylacetic acid; the MS culture medium is prepared from 50 ml / L macroelements, 5 ml / L microelements, 5 mg / L ferric salt, 5 mg / L organic ingredients, 30 g / L saccharose and 6.5 g / L agar, the macroelements comprise 1,900 mg / L potassium nitrate, 1,650 mg / L ammonium nitrate, 170 mg / L monopotassium phosphate, 370 mg / L magnesium sulfate and 440 mg / L calcium chloride; the microelements comprise 0.83 mg / L potassium iodide, 6.2 mg / L boric acid, 22.3 mg / L manganese sulfate, 8.6 mg / L zinc sulfate, 0.25 mg / L sodium molybdate, 0.025 mg / L copper sulfate and 0.025 mg / L cobalt chloride; ferric salt comprises 37.3 mg / L ethylene diamine tetraacetic acid and 27.8 mg / L ferrous sulfate; the organic ingredients comprise 100 mg / L inositol, 2 mg / L glycine, 0.1 mg / L thiamine hydrochloride, 0.5 mg / L pyridoxine hydrochloride and 0.5 mg / L niacin. The tissue culture medium and culture method have the advantages that the rooting capacity of the malus spectabilis adopting the culture method is high, the emergence rate of 100% is guaranteed, and mass malus spectabilis can be cultured; the malus spectabilis with mutation characteristics can be cultured, and the mutation characteristics are kept.

Description

technical field [0001] The invention belongs to the technical field of nursery stock cultivation, and in particular relates to a tissue culture medium and a cultivation method of winter red fruit. Background technique [0002] Winter red fruit ( M. domestica cv. Winter Red Fruit) is a small tree with green leaves and deciduous leaves belonging to Rosaceae apple. The fruit is in clusters, usually green in spring and summer, and then gradually turns yellow, until the fruit color is bright red in late autumn, until the next spring. It is small, crystal clear and colorful. Therefore, winter Hongguo has become an excellent tree species for cultivating fruit-watching bonsai. The bright red color of the fruit is best seen around the Spring Festival, and its bright color, which symbolizes auspiciousness, harvest and celebration, is especially popular among Chinese consumers and has great market potential. However, as a kind of bonsai class ornamental plant, the green leaves and gr...

AI Technical Summary

Problems solved by technology

[0005] The invention provides a tissue culture medium and a culture method of Donghong fruit, uses biotechnology combined with conventional breeding methods to preserve the germplasm resources of the mutation characteristic traits of Donghong fruit, and develops a meristematic medium formula for tissue culture seedlings to solve the current problem There are technical problems

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