Cymbidium-ensifolium tissue-culture rapid propagation method

Abstract

The invention relates to the technical field of plant tissue culturing, in particular to a cymbidium-ensifolium tissue-culture rapid propagation method. The cymbidium-ensifolium tissue-culture rapid propagation method includes the following steps that plant pretreating, material selecting, material sterilizing, induction culturing, cluster-bud subculture multiplication culturing, rooting and hardening culturing and transplanting. According to the cymbidium-ensifolium tissue-culture rapid propagation method, as explant and the sterilization method are adopted, the induction rate of the explant can be greatly increased, and meanwhile cultured seedlings are high in survival seedling rate and good in growth vigor; the commercialized production requirements are met in a short time with the biotechnology means, and the technical support is provided for cymbidium-ensifolium large-scale and industrialization culturing in enterprises.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a rapid propagation method of Jianlan tissue culture. Background technique [0002] Jianlan is a terrestrial plant that grows in sparse forests, bushes, valleys or grass at an altitude of 600-1800 meters. Produced in Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Hunan, Guangdong, Hainan, Guangxi, southwestern Sichuan, Guizhou and Yunnan in China. Widely distributed in Southeast Asia and South Asia, north to Japan. At present, most of the propagation methods used are branch propagation, and few seeds are formed, and the development of seeds is relatively difficult. Basically, it is relatively difficult to germinate under natural conditions. Therefore, the main means of efficient propagation of Jianlan is tissue culture. Some developed countries in foreign countries adopt industrial seedling breeding technology and tissue culture rapid propagation technology, which can n...

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Problems solved by technology

Some developed countries in foreign countries adopt industrial seedling breeding technology and tissue culture rapid propagation technology, which can not only create huge economic benefits, but also cultivate many excellent varieties. However, the research on the rapid propagation technology of Jianlan tissue culture in my country is still in its infancy. To a large extent hindered the large-scale production of Jianlan

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