Malus hupehensis and Malus micromalus Makino seed refrigerator lamination method

Abstract

The invention provides a Malus hupehensis and Malus micromalus Makino seed refrigerator lamination method. The method comprises the following steps: selecting healthy and plum Malus hupehensis and Malus micromalus Makino seeds collected in autumn, immersing the selected seeds in a 1.5% sodium hypochlorite solution for 15 min, airing the immersed seeds, processing the seeds at three different lamination times, putting a matrix block in an aperture disk, thoroughly watering the matrix block in the laminating process twice, sowing the seeds in the matrix block, laminating the seeds in a 0 DEG C refrigerator, taking out the seeds in the next spring, carrying out germination rate statistics, and determining the optimal lamination time according to the germination rate in order to construct Malus hupehensis and Malus micromalus Makino seed refrigerator lamination technology standards and select the time with high emergence rate as the determined lamination time. The method has the advantages of convenience in operation, controllable conditions, standardized and consistent seedling emergence, high emergence rate, and convenience for apple stock test and high-efficiency lamination of seeds in the factory seedling growing process, and is suitable for being applied as a stock seed refrigeration lamination method.

Description

technical field [0001] The invention relates to a layering technology of apple rootstock Pingyi sweet tea and baleng crabapple seeds, in particular to a cold storage layering method for Pingyi sweet tea and baleng crabapple seeds. Background technique [0002] Apple is a vegetatively propagated plant, and the planting of varieties needs to be grafted onto rootstocks. The main rootstocks of apple varieties in my country include Vitex, Pingyi Sweet Tea, Baleng Haitang, Xinjiang Wild Apple, etc. Among them, Pingyi Sweet Tea and Baleng Haitang are due to Its strong stress resistance is widely used as the base stock of arborization stock or dwarfing middle stock in the main apple producing areas in my country. The selection and basic research of stress-resistant root stock and ear combination of Pingyi sweet tea and baleng crabapple has always been a priority in my country and even in the world. The focus of world apple rootstock research. [0003] The stratification of apple root...

AI Technical Summary

Problems solved by technology

[0003] The stratification of apple rootstocks in my country has always been dominated by sand storage in cellars. The advantage is that it is easy to operate and is suitable for a large number of seed stratification. The disadvantage is that the stratification temperature is uncontrollable, and the seeds will be lost during the separation process of sand and rootstock after stratification. It is easy to knock off the seed buds when the germinated seeds are separated from the sand and sown

This stratification method is acceptable for large-scale field production, but for scientific research in the laboratory, this method has serious losses, lacks rigor, and is less practical