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A curd-shaped co3(po4)2/chloroperoxidase hybrid nanoreactor and its application

A technology of chloroperoxidase and chloroperoxide, which is applied in the direction of oxidoreductase, chemical instruments and methods, enzymes, etc., can solve problems such as application obstacles and poor stability, and achieve improved tolerance, good compactness, The effect of increasing the contact area

Inactive Publication Date: 2018-10-23
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, despite the above advantages, the large-scale industrialization of the enzyme is due to the poor stability of the enzyme for a long time in the actual reaction environment such as high temperature, strong acid and strong alkali, and the presence of organic solvents, and it is difficult to separate from the reaction system and reuse it. applications are blocked

Method used

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  • A curd-shaped co3(po4)2/chloroperoxidase hybrid nanoreactor and its application
  • A curd-shaped co3(po4)2/chloroperoxidase hybrid nanoreactor and its application
  • A curd-shaped co3(po4)2/chloroperoxidase hybrid nanoreactor and its application

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Experimental program
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Embodiment 1

[0021] Add 25μL 0.2mmol / L chloroperoxidase aqueous solution and 600μL 0.1mmol / L cobalt acetate aqueous solution to 9.375mL0.1mol / L phosphate buffer solution with a pH value of 5.8, mix well, and the chloroperoxidase in the resulting mixture The concentration of cobalt acetate is 0.5μmol / L, the concentration of cobalt acetate is 6mmol / L, and then the resulting mixture is placed in a constant temperature incubator at 20°C for 3 days, then the supernatant is removed, and then placed in a vacuum oven at 32°C dried under vacuum to obtain flower-like Co 3 (PO 4 ) 2 / chloroperoxidase hybrid nanoreactor.

Embodiment 2

[0026] Add 25μL 0.1mmol / L chloroperoxidase aqueous solution and 500μL 0.1mmol / L cobalt acetate aqueous solution to 9.475mL0.1mol / L phosphate buffer solution with a pH value of 3, mix well, and the chloroperoxidase in the resulting mixture The concentration of cobalt acetate is 0.25μmol / L, the concentration of cobalt acetate is 5mmol / L, and then the resulting mixture is placed in a constant temperature incubator at 20°C for 3 days, then the supernatant is removed, and then placed in a vacuum oven at 32°C dried under vacuum to obtain flower-like Co 3 (PO 4 ) 2 / chloroperoxidase hybrid nanoreactor, wherein the immobilized amount of chloroperoxidase is 45.7mg / g.

Embodiment 3

[0028]Add 30 μL of 0.2 mmol / L chloroperoxidase aqueous solution and 900 μL of 0.1 mmol / L cobalt acetate aqueous solution to 9.070 mL of 0.1 mol / L phosphate buffer solution with a pH value of 5, mix well, and the chloroperoxidase in the resulting mixture The concentration of cobalt acetate is 0.6μmol / L, the concentration of cobalt acetate is 9mmol / L, and then the resulting mixture is placed in a constant temperature incubator at 20°C for 3 days, then the supernatant is removed, and then placed in a vacuum oven at 32°C dried under vacuum to obtain flower-like Co 3 (PO 4 ) 2 / chloroperoxidase hybrid nanoreactor, wherein the immobilized amount of chloroperoxidase is 50.6mg / g.

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Abstract

The invention relates to a ball-flower-shaped Co3(PO4)2 / chloroperoxidase hybridized nanoreactor and an application thereof. The ball-flower-shaped Co3(PO4)2 / chloroperoxidase hybridized nanoreactor, which is relatively small in diameter, relatively good in compactness and stable in structure, can be obtained through adding chloroperoxidase and cobalt acetate into a phosphate buffer solution with the pH value of 3 to 6, standing the obtained mixed solution, removing supernatant, and carrying out vacuum drying. A preparation method of the ball-flower-shaped Co3(PO4)2 / chloroperoxidase hybridized nanoreactor is simple, and the prepared ball-flower-shaped Co3(PO4)2 / chloroperoxidase hybridized nanoreactor is good in thermal stability and high in tolerance to reaction environments; the total amount of the chloroperoxidase exposed to surface is relatively large, so that the area of contact of the chloroperoxidase with a substrate during reaction is increased; during the degradation of triphenylmethane water-soluble dyes, relatively high catalytic activity is shown, and the rate of degradation to crystal violet in five minutes can reach 80% or more, so that the ball-flower-shaped Co3(PO4)2 / chloroperoxidase hybridized nanoreactor has a relatively good application prospect.

Description

technical field [0001] The invention belongs to the technical field of enzyme immobilization, in particular to a Co 3 (PO 4 ) 2 / Chloroperoxidase hybrid nanoreactor and its synthesis method and its application in catalytic degradation of triphenylmethane water-soluble dye crystal violet. Background technique [0002] Enzymatic reactions have many advantages, such as: mild reaction conditions (normal temperature and pressure and pH close to physiological conditions), fast reaction, good selectivity, etc., and can avoid complex functional group protection or activation steps in organic reactions, so It is more efficient, more economical and greener than general organic synthesis. However, despite the above advantages, the large-scale industrialization of the enzyme is due to the poor stability of the enzyme for a long time in the actual reaction environment such as high temperature, strong acid and strong alkali, and the presence of organic solvents, and it is difficult to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C02F3/34C02F101/38
CPCC02F3/342C02F2101/308C02F2101/38C12N9/0065C12N11/14C12Y111/01
Inventor 蒋育澄樊雪婷胡满成李淑妮翟全国
Owner SHAANXI NORMAL UNIV
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