Imaging device and method for rapidly obtaining three-dimensional structure information and molecular phenotype information of large samples

Abstract

An imaging device and method for quickly acquiring large-sample three-dimensional structure information and molecular phenotype information. The apparatus comprises: a sample storage device; a three-dimensional mobile station (9) for driving the sample storage device to move within a three-dimensional space; a vibration slicing module (8) for slicing the sample (5) so that the sample obtains a superficial-layer portion; and a wide-field optical microimaging module for performing high-throughput tomography on the superficial-layer portion of the sample. By means of the device and method, the disadvantages in the prior art that a sample preparation flow is complex, a sample form and a fluorescence signal are affected and an imaging speed is slow are solved, and three-dimensional structure information about a sample can be quickly acquired and analyzed, wherein the acquired data has an auto-registration characteristic, molecular phenotype dyeing is performed on a sample slice in a position of interest to obtain molecular phenotype information about the sample, and same can be registered into the acquired three-dimensional structure information.

Description

technical field [0001] The invention relates to microscopic imaging, in particular to an imaging device and method for rapidly acquiring three-dimensional structure information and molecular phenotype information of large samples. Background technique [0002] The brain is one of the most complex systems in nature, governing all human activities. The exploration of the brain has always been the goal of human research, but until now we still cannot accurately describe the mechanism of intelligence, thinking and consciousness. Neural structure is an important basis for the realization of advanced brain functions. Complex brain functions require the participation of multiple brain regions and are coordinated by local and long-range neural circuits. Brain diseases are often accompanied by structural abnormalities in functionally related specific neural circuits and their input connections and output projection relationships. In order to decipher the structural basis of brain ...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a device and method for quickly obtaining three-dimensional structure information and molecular phenotype information of large samples. Due to the shortcomings of slow signal and imaging speed, the three-dimensional structure information of the sample can be quickly obtained and analyzed. The obtained data has the characteristics of self-registration, and the molecular phenotype staining is performed on the sample slices of the interested part to obtain the molecular phenotype information of the sample. And can be registered to the acquired 3D structural information

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