Cell phenotype based high-content multi-index renal toxicity detection method and applications thereof

A detection method and technology of nephrotoxicity, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., to achieve obvious effects, convenient operation, and comprehensive evaluation indicators

Inactive Publication Date: 2016-09-07
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0003] At present, there is no relevant literature report on the application of high-content detection technology to the evaluation of nephrotoxicity. In view of this, the high-content detection method is applied to the detection of nephrotoxic drugs to evaluate whether the drug has nephrotoxicity, and try to apply this method to A variety of potential nephrotoxic monomeric components of traditional Chinese medicine were detected in the literature to verify the sensitivity and accuracy of the method, thus providing more possibilities for a faster, more cost-effective evaluation of the safety of traditional Chinese medicine

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  • Cell phenotype based high-content multi-index renal toxicity detection method and applications thereof
  • Cell phenotype based high-content multi-index renal toxicity detection method and applications thereof
  • Cell phenotype based high-content multi-index renal toxicity detection method and applications thereof

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[0031] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0032] The present invention provides a high-content multi-indicator nephrotoxicity detection method based on cell phenotype, comprising: using fluorescent molecular probes capable of specifically detecting changes in cell nucleus or mitochondrial function to mark cells, and using a high-content screening system As a detection method, based on the high-content screening system, imaging analysis is performed on living cells that have been added with the drug to be tested, and at least one effect of the drug to be tested on the number of cells, nuclei, and mitochondria is obtained, so as to determine the impact on cells.

[0033] In another embodiment, the drug to be tested includes: aristolochic acid A, cyclosporine A, and cisplatin, and the calculated detection concentration i...

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Abstract

The invention discloses a cell phenotype based high-content multi-index renal toxicity detection method and applications thereof. According to the detection method, A fluorescent molecular probe that can change the functions of nucleus or mitochondrial and carry out specific detection is adopted to label cells; a high content screening system is taken as the detection means, based on the high content screening system, live cells, which are injected with drugs to be detected, are subjected to imaging analysis so as to determine at least one influence of the drugs on cell number, nucleus and mitochondrial, and thus the influence of the drugs on the cells can be determined. The invention also discloses applications of the detection method. The provided detection method has the advantages that multiple indexes can be detected, while the cell structure and functional integrity are maintained, and the detection method has the characteristics of rapidness, high flux, low cost, and high accuracy.

Description

technical field [0001] The invention relates to the field of drug toxicity detection, in particular to a cell phenotype-based high-content multi-index nephrotoxicity detection method and its application. Background technique [0002] Chinese herbal medicine has been used clinically in my country for more than 2,000 years, but the research on its toxic and side effects has just started. Drug toxicity is an adverse reaction that often occurs during the development and clinical use of Chinese medicine. Due to the lack of people's understanding of the safety of traditional Chinese medicine, the number of drug-induced injuries caused by self-administered, long-term, large-scale, and blind use of Chinese herbal medicines has increased year by year. The kidney is extremely vulnerable to adverse reactions due to its functions and unique blood circulation characteristics. organ. The research on the early nephrotoxicity of drugs mainly uses animal or human and animal kidney cells as ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 朱彦王萌任晓亮高秀梅崔英姜苗苗王晓明邵瑞郭晓张慧杰谭乐俊
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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