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Preparation method of silkworm chrysalis-cordyceps militaris fermentation product feed additive

A technology of feed additives and fermented products, which is applied in the field of preparation of feed additives fermented by Cordyceps militaris, can solve the problems of poor health care efficacy, single nutrition, unsatisfactory, etc., achieve high nutritional value, improve immunity, and have no toxic side effects

Inactive Publication Date: 2016-08-24
LIANYUNGANG HENGTU FARMING DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The feed additives in the prior art have single nutrition, poor health care effect, and cannot meet the needs of actual use

Method used

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  • Preparation method of silkworm chrysalis-cordyceps militaris fermentation product feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of preparation method of Cordyceps militaris fermentation product feed additive that the present invention proposes, comprises the following steps:

[0020] S1. Inoculate the refrigerated Cordyceps militaris strains into flat plate solid medium, cultivate them in a constant temperature incubator at 25°C for 6.5 days to obtain activated strains, then cultivate the strains at 25°C with a humidity of 96% for 14 days to obtain strains, and then Inoculate the bacterial strain into the liquid seed culture medium, the shaker temperature is 25°C, and cultivate at 120r / min for 7 days to obtain the liquid mother seed. The raw materials of the solid fermentation medium include by weight: 10 parts of cottonseed hulls, 5 parts of wheat bran, corn 4 parts of flour, 4 parts of potato, 4.5 parts of sucrose, 3 parts of peptone, 2.5 parts of potassium dihydrogen phosphate, 3.5 parts of magnesium sulfate, 4 parts of glycine, and 3 parts of gibberellin; Put it in an artificial incub...

Embodiment 2

[0024] A kind of preparation method of Cordyceps militaris fermentation product feed additive that the present invention proposes, comprises the following steps:

[0025] S1. Inoculate the refrigerated Cordyceps militaris bacterial strain into flat plate solid medium, cultivate in a constant temperature incubator at 20°C for 8 days to obtain activated strains, then cultivate the strains at 24°C with a humidity of 97% for 12 days to obtain bacterial strains, and then The bacterial strain is inoculated into the liquid seed medium, the shaker temperature is 26°C, and the liquid mother seed is obtained by culturing at 110r / min for 8 days. The raw materials of the solid fermentation medium include by weight: 5 parts of cottonseed hulls, 8 parts of wheat bran, and corn flour 3 parts, 6 parts of potato, 3 parts of sucrose, 4 parts of peptone, 1 part of potassium dihydrogen phosphate, 5 parts of magnesium sulfate, 3 parts of glycine, 4 parts of gibberellin; then evenly inoculate the li...

Embodiment 3

[0029] A kind of preparation method of Cordyceps militaris fermentation product feed additive that the present invention proposes, comprises the following steps:

[0030] S1. Inoculate the refrigerated Cordyceps militaris bacterial strain into flat plate solid medium, and cultivate it in a constant temperature incubator at 30°C for 5 days to obtain activated strains, and then culture the strains at 26°C with a humidity of 95% for 16 days to obtain bacterial strains, and then The strain was inoculated into the liquid seed medium, the temperature of the shaker was 24°C, and the liquid mother seed was obtained by culturing at 130r / min for 6 days. The raw materials of the solid fermentation medium included by weight: 15 parts of cottonseed hulls, 2 parts of wheat bran, and corn flour 5 parts, 2 parts of potato, 6 parts of sucrose, 2 parts of peptone, 4 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 5 parts of glycine, 2 parts of gibberellin; then evenly inoc...

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Abstract

The present invention provides a preparation method of silkworm chrysalis-cordyceps militaris fermentation product feed additive. The preparation method comprises the following steps: S1, cordyceps militaris strains are inoculated into a plate solid medium, the incubated cordyceps militaris strains are cultivated at a constant temperature to obtain the activated cordyceps militaris strains, and after the cordyceps militaris strains are obtained by the cultivation, the cordyceps militaris strains are inoculated into a liquid seed medium, and liquid mother strains are obtained by a shaker cultivation; then the liquid mother strains are inoculated onto a solid fermentation medium, the inoculated mother strains are placed into an artificial incubator to conduct cultivation to obtain a cordyceps militaris fermention product, and the cordyceps militaris fermention product is dried to obtain a material a; S2, corn flour, soybean meal, rhizoma atractylodis macrocephalae, poria cocos and tea polyphenols are added into the material a to be mixed evenly, then water and aspergillus niger are added, and the mixture is fermented to obtain a material b; and S3, bone meal, vitamin C, vitamin E, methionine, lysine and probiotics are added to obtain the silkworm chrysalis-cordyceps militaris fermentation product feed additive. The silkworm chrysalis-cordyceps militaris fermentation product feed additive has functions of promoting growth, preventing oxidation, improving immune, etc., is free of toxic or side effects, and is high in nutritional value.

Description

technical field [0001] The invention relates to the technical field of feed additives, in particular to a preparation method of a feed additive from fermented Cordyceps militaris. Background technique [0002] At present, silkworm chrysalis is the chrysalis of the Bombyx mori family insect, which can be used as feed and traditional Chinese medicine, and has the effects of harmonizing the spleen and stomach, dispelling rheumatism, and growing yang. Modern scientific research shows that dried silkworm chrysalis contains about 55% to 60% of protein, 25% to 30% of fatty acid, carbohydrates, chitin, various protein hormones, multiple vitamins, and various trace elements. It has high nutritional and medicinal properties. Use development value and market prospect. Silkworm chrysalis protein is a high-quality pure natural full-price protein, which can be called the first in nutrition. Silkworm chrysalis protein contains 18 kinds of amino acids, the composition is reasonable and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K10/30A23K10/37A23K10/26A23K20/174A23K20/142A23K10/35A23K20/26
CPCY02P60/87
Inventor 张亮杨海蓉宋兆霞
Owner LIANYUNGANG HENGTU FARMING DEV CO LTD
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