Method for in-situ and real-time detection of concentration of hydrogen peroxide in living cells

Abstract

The invention provides a method for in-situ and real-time detection of the concentration of hydrogen peroxide in living cells. The method comprises the following steps: carrying out electrochemical test to obtain the current-time curve of the living cells stimulated by a medicine, carrying out electron paramagnetic resonance wave spectrum test to obtain the ESR map of the living cells irradiated by ultraviolet lights in an electrochemical cell, and establishing an ESR signal intensity-current relation curve; and carrying out electron paramagnetic resonance wave spectrum test on a hydrogen peroxide standard solution to obtain the ESR map of the hydrogen peroxide standard solution irradiated by the ultraviolet lights, establishing an ESR signal intensity-hydrogen peroxide concentration standard curve, and synthesizing above three curves to obtain the concentration of hydrogen peroxide released by the stimulated living cells at any time in order to complete real-time detection of the concentration of hydrogen peroxide in the living cells. The method allows the quantitative detection of the concentration of the hydrogen peroxide released by the living cells to be accurate, can realize in-situ and real-time monitoring of the concentration of hydrogen peroxide released by living cells, and has wide application prospect.

Description

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AI Technical Summary

Benefits of technology

This patented inventor describes an improved way to measure how much oxygen gas reacted inside live tissues during metabolism or other processes like blood flowing through them. By combining both techniques together they make it possible to accurately determine what chemical substances are being released from within each part of their body. These combined technologies allow researchers to monitor changes caused by certain compounds on specific parts of our bodies over time without having to use expensive equipment.

Problems solved by technology

There currently exist various techniques for measuring free radical (representatives) production during aerobic activities like nitrogen monooxygenase-2(NMO2) producing cyclohexaimides, iron chloroperoxyradium complexes, catalysts containing metal atoms, proteins involved in energy transfer processes within our bodies, and lipids associated with neurons. These chemical substances may affect vital functions inside these organs through interactions with specific binding sites called histones and enolysers found throughout them. Current analytical tools require long hours to measure concentrations accurately while still being invasively applied to live systems.

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