Kit for determining glycated serum proteins and preparation method of kit

Abstract

The invention discloses a kit for determining glycated serum proteins and a preparation method of the kit. The kit comprises two liquid components of a reagent R1 and a reagent R2 which are independent from each other, and comprises the following components of the following content: the reagent R1: 10 to 90 mmol / L of potassium phosphate buffer solution, 50 to 250 mmol / L of calcium chloride, 3 to 9 KU / L of uricase, 10 to 18 KU / L of ascorbic acid oxidase, 2.0 to 12.0 g / L of alkyl glycoside, 0.3 to 0.7 mmol / L of TritonX-100, 0.01 to 0.09 g / L of sodium azide, and a solvent of the reagent R1 is purified water; the reagent R2: 10 to 90 mmol / L of potassium phosphate buffer solution, 0.3 to 1.5 mmol / L of blue tetrazolium, 0.01 to 0.09 g / L of sodium azide, and a solvent of the reagent R2 is purified water. The preparation method comprises the following steps: preparing the reagents according to the component content; mixing a sample to be detected, the reagent R1 and the reagent R2, and enabling the mixture to sufficiently react; determining an absorbance difference value after the reaction by utilizing a full-automatic biochemical analyzer; calculating the concentration of the glycated serum proteins in the sample according to an absorbance variation value. The kit has the advantages of high accuracy, no pollution and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring glycosylated serum protein and a preparation method thereof. Background technique [0002] Glucose in the blood undergoes a non-enzymatic glycation reaction with the N-terminus of albumin and other protein molecules to form glycated serum proteins. The glycation of serum proteins mainly occurs on the lysine residues of protein molecules, of which albumin is the main one, and glycated serum The half-life of protein (GSP) is 17-19 days. The measurement of glycosylated serum protein (GSP) can reflect the average blood sugar level of 2-3 weeks before the measurement, and is not affected by the blood sugar concentration at that time. It is a good indicator for monitoring blood sugar in diabetic patients. Therefore, the determination of glycosylated serum protein is of great significance in the diagnosis, short-term monitoring and efficacy evaluat...

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a test kit for the determination of glycosylated serum protein in order to overcome the defects of radiochemical method with radioactive pollution, gas chromatography with complicated operation and long time consumption, and enzymatic method with poor reagent stability and its preparation method

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