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A method for increasing the biomass and acid production of lactic acid bacteria

A technology of lactic acid bacteria and biomass, applied in the field of bioengineering, can solve the problems of high cost, long production cycle, shortened fermentation time, etc.

Active Publication Date: 2020-03-31
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on improving the production of lactic acid bacteria mainly focuses on the research of the culture medium, such as the patent of a new culture medium that is conducive to improving the production of lactic acid bacteria, application number 201510487910.1, a fermentation medium of lactic acid bacteria and a high-yield production process of lactic acid, application number CN201510516297.1 , using the traditional method of improving the medium and process, although the yield can be increased, the production cycle is long and the cost is high. In view of this, the study uses ultrasonic means to assist the fermentation to increase the number and yield of lactic acid bacteria, shorten the fermentation time, and reduce production. cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Preparation of Medium

[0020] Weigh 7.5 g of peptone, 7.5 g of yeast powder, 10.0 g of glucose, 100 mL of tomato juice, 2.0 g of potassium dihydrogen phosphate, 0.5 mL of Tween 80, and 900 mL of distilled water, adjust the pH to 7.0, and sterilize at 120°C for 20 minutes.

[0021] 2. Preparation of Seed Solution

[0022] Add a ring of lactic acid bacteria strains preserved at 4°C to each bottle in the liquid medium ( Lactobacillus , China Agricultural Microorganism Collection Center, ACCC10637), cultivated at 37°C, 150 r / min, for 16 h.

[0023] 3. Lactic acid bacteria fermentation culture

[0024] Take 10% of the seed solution into the culture medium, ferment at 37°C with a shaker speed of 150 r / min, take it out after 1 hour of fermentation, and place it in an ultrasonic instrument with an ultrasonic frequency of 40 kHz for 4 hours. min, the ultrasonic power was 200W, and after the ultrasonic was completed, the fermentation was continued for 12 h in a shaker at a...

Embodiment 2

[0028] 1. Preparation of Media

[0029] Weigh 10.0 g of peptone, 8.0 g of beef extract, 4.0 g of yeast powder, 20.0 g of glucose, 0.2 g of magnesium sulfate, 5.0 g of sodium acetate, 2.0 g of diammonium citrate, 2.0 g of dipotassium hydrogen phosphate, 0.05 g of manganese sulfate, and Warm 80 1.0 mL, distilled water 1000 mL, adjust the pH to 6.0, and sterilize at 120°C for 20 minutes.

[0030] 2. Preparation of Seed Solution

[0031] Add a ring of lactic acid bacteria strains preserved at 4°C to each bottle in the liquid medium ( Lactobacillus plantaru , China Industrial Microorganism Collection Center, CICC20871), at 35°C, 180 r / min, cultured for 12 h.

[0032] 3. Lactic acid bacteria fermentation culture

[0033] Take 5% of the seed solution into the culture medium, ferment at 35°C with a shaker speed of 180 r / min, take it out after 3 hours of fermentation, and place it in an ultrasonic instrument with an ultrasonic frequency of 28 kHz for 10 min, the ultrasonic power w...

Embodiment 3

[0037] 1. Preparation of Media

[0038] Weigh 10.0 g of peptone, 10.0 g of beef extract, 5.0 g of yeast powder, 5.0 g of glucose, 5.0 g of sodium acetate, 2.0 g of diamine citrate, 1.0 mL of Tween 80, 2.0 g of dipotassium hydrogen phosphate, and 0.2 g of magnesium sulfate heptahydrate. g, 0.05 g of manganese sulfate monohydrate, 20.0 g of calcium carbonate, 1000 mL of distilled water, adjust the pH to 6.8, and sterilize at 120°C for 20 minutes.

[0039] 2. Preparation of Seed Solution

[0040] Add a ring of lactic acid bacteria strains preserved at 4°C to each bottle in the liquid medium ( Lactococcus lactis , China General Microorganism Culture Collection and Management Center, CCGMC1.62), cultivated at 30°C, 130 r / min, for 14 h.

[0041] 3. Lactic acid bacteria fermentation culture

[0042] Take 20% of the seed solution and put it into the medium, ferment at 30°C with a shaker speed of 130 r / min, take it out after 2 hours of fermentation, and place it in an ultrasonic in...

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Abstract

The invention discloses a method for increasing lactic acid bacterium biomass and the acid yield and relates to the field of bioengineering. The lactic acid bacterium biomass and the acid yield are increased by adopting ultrasound as a supplementary means in the lactic acid bacterium fermentation process. With the adoption of the method, the lactic acid bacterium biomass is increased by 12%-55%, the acid yield is increased by 15%-60%, the time for reaching the highest acid yield is shortened by 0.5-3 h, the fermentation time can be shortened, and the production cost can be reduced.

Description

technical field [0001] The invention relates to the technical field of bioengineering, and uses ultrasound as an auxiliary means to increase the biomass and acid production of lactic acid bacteria. After adopting this method, the biomass of lactic acid bacteria increased by 12% to 55%, the acid production increased by 15% to 60%, and the time to reach the maximum acid production was shortened by 0.5 to 3 hours. Background technique [0002] Lactic acid bacteria belong to Gram-positive bacteria, which are a type of microorganisms that can convert lactose into lactic acid and reduce the pH value of fermented products. As a food-safe microorganism, lactic acid bacteria are widely used in various foods such as fermented yogurt, lactic acid bacteria drinks, pickles, cheese, sauce, soy sauce and bread. In addition, lactic acid bacteria have various functions such as promoting the decomposition and absorption of nutrients, lowering cholesterol, inhibiting the proliferation of path...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/40C12R1/225C12R1/01
CPCC12N1/20C12P7/40
Inventor 钱静亚吕复强任珊珊马海乐
Owner JIANGSU UNIV
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