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Application of water-soluble extract of spirodela polyrrhiza in promoting denitrifying activity of denitrifying microorganisms

A technology of water-soluble extracts and denitrifying microorganisms, which is applied in the field of control of nitrate-polluted water bodies, can solve problems such as the absence of active extracts of duckweed, so as to promote the denitrification activity of denitrifying microorganisms, avoid water quality deterioration, The effect of enhancing processing efficiency

Active Publication Date: 2016-07-20
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the application of duckweed water-soluble extract as a signal to promote denitrification activity has not been reported, and there is no technology to prepare active extract from purple-backed duckweed.

Method used

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  • Application of water-soluble extract of spirodela polyrrhiza in promoting denitrifying activity of denitrifying microorganisms
  • Application of water-soluble extract of spirodela polyrrhiza in promoting denitrifying activity of denitrifying microorganisms
  • Application of water-soluble extract of spirodela polyrrhiza in promoting denitrifying activity of denitrifying microorganisms

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Embodiment 1

[0019] 1. Preparation of water-soluble extract of duckweed purple back

[0020] The water-soluble extract of Duckweed is obtained through the following steps:

[0021] (1) Take the freeze-dried Duckweed, grind it into fine powder, weigh about 0.50g into a 150mL Erlenmeyer flask, add 50mL of methanol / water mixture (volume ratio 4:1), shake at 20°C and 120rpm Extraction under bed conditions for 16h. The extract was vacuum filtered through a WhatmanGF / F glass fiber membrane, the filtrate was then rotary evaporated at 35°C to remove methanol, and the remaining aqueous solution was freeze-dried to obtain the total fraction of the extract.

[0022] (2) For separation steps, see figure 1 , dilute the total components of the extract obtained in (1) to 50mL with water, adjust the pH to about 6.5, extract 50mL of ethyl acetate three times, and combine the extracts to obtain the fat-soluble neutral component of the extract; use 1mol / L HCl Adjust the pH of the remaining solution to 2.0...

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Abstract

The invention discloses application of a water-soluble extract of spirodela polyrrhiza in promoting denitrifying activity of denitrifying microorganisms. The invention discloses the new application of the water-soluble active extract of spirodela polyrrhiza in promoting the denitrifying activity of denitrifying microorganisms on the research basis of original chemical components and traditional effects of the spirodela polyrrhiza, and provides a novel purifying synergistic agent for treating nitrate-polluted water bodies in environmental protection. Simultaneously, the application has the advantages that wastes become valuables, the treatment efficiency of nitrate-polluted water bodies is enhanced by utilizing the spirodela plant extract according to local situation, and the problem of water-quality deterioration caused by floating of a great amount of spirodela polyrrhiza in rivers and lakes is effectively avoided. In addition, the invention provides a plant-sourced green promoter for removing nitrates. The plant-sourced green promoter does not contain an organic solvent, mainly plays a role in regulating and controlling signals, is environmental-friendly, safe and non-toxic, is low in cost and provides a new method and idea for constructing a bioecological technology for treating nitrate-polluted water bodies by high efficiency.

Description

technical field [0001] The invention belongs to the research field of control technology of nitrate-polluted water bodies, and in particular relates to a preparation method of a water-soluble extract of duckweed and its application in promoting the denitrification activity of denitrifying microorganisms. Background technique [0002] The first national survey of pollution sources in 2010 showed that nitrate pollution from agricultural sources has surpassed industrial sources to become the main source of total nitrogen pollution in water bodies in my country, causing eutrophication of rivers and lakes, leading to surface water pollution and excessive nitrate in groundwater. , a serious threat to human health. From a long-term perspective, excessive nitrate will lead to changes in the structure and diversity of the entire ecological community. Therefore, the purification treatment of agricultural wastewater and fertilizer water has become a new research hotspot in the field of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C02F3/34C12R1/38C02F101/16
CPCC02F3/34C02F2101/163C12N1/20C12N1/38
Inventor 陆玉芳施卫明孙力
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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