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Method for amorphophallus virosus crossbreeding

A technology for hybrid breeding of konjac verruca is applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as failure to exert effect, short flowering period, and ineffective flowering, and achieves high seed setting rate and low cost. , the effect of less virus accumulation

Active Publication Date: 2016-07-20
DEHONG DAI JINGPO AUTONOMOUS PREFECTURE AGRI TECH PROMOTION CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Amorphophallus verrucosum described in the present invention has the advantages of resistance to konjac soft rot and high yield, and is a very rare excellent germplasm resource in the improvement of konjac varieties. The fruit is strong, which has led to the current academic mistakenly believe that Amorphophallus verrucosum is "flowers but not fruit", so that its role in Amorphophallus konjac hybrid breeding has not been brought into play

Method used

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  • Method for amorphophallus virosus crossbreeding
  • Method for amorphophallus virosus crossbreeding
  • Method for amorphophallus virosus crossbreeding

Examples

Experimental program
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Effect test

Embodiment 1

[0046] In 2010, 26 flowering plants of Amorphophallus konjac were cultivated in the experimental site of Dehong Prefecture Agricultural Science Institute according to technical step A (the same below); artificial auxiliary pollination was carried out according to technical step E (the same below), and 1 plant was successfully induced to bear fruit. ~6 weeks later, the scapes are as high as 91 cm, the appendages are separated from the mother body, and the later growth is normal; the remaining 25 plants will yellow and wither after 2 weeks of pollination, and the appendages will stay; in late December, the berries of the surviving plants are fully mature and red, According to technical step F (the same below), the seeds were harvested in batches and dried on the spot; a total of 418 berries were harvested. The thousand-grain weight of the berries was 286g, and each berry contained 1 to 2 seeds. In March of the following year, the seedlings were sowed according to technical step G ...

Embodiment 2

[0048] In 2011, 21 flowering plants of Amorphophallus konjac were cultivated; pollen vigor was measured according to technical step (B, the same below); pollination was carried out according to technical step (C1), and the pollination time was between 19:00 and 21:00 (below Same), respectively number the observation records (see Table 1); after artificial pollination, 4 plants bear fruit, and the material numbers are: 4, 5, 8, 20; the remaining plants yellow and wither after 2 weeks; the surviving plants in late December The berries are mature and the appendages stay in place. The seeds are harvested in batches and air-dried on the spot. A total of 1,412 berries are harvested, with a thousand-grain weight of 289g (see Table 1). Seedlings were sown in March of the following year, and 2125 seedlings were obtained; in mid-May, the field was transplanted with a survival rate of 88.3%; in mid-November, the plants fell after the seedlings were harvested and dug, and a total of 402.8 k...

Embodiment 3

[0052] In 2012, 57 flowering plants of Amorphophallus konjac were cultivated. Measure pollen vigor according to technical step (B); the first batch of flowering plants did not bear fruit after same pollination; 40 plants were selected for artificial assisted pollination experiment according to technical step (C1), with bracts spreading 60%, 80%, 100% ("Trumpet-shaped") and four treatments during the powder-spreading stage, each with 10 plants. The experimental results are shown in Table 2, the fruit hanging rate = the number of full fruit / the number of fruit. The test results show that Amorphophallus wartii pollination does not bear fruit; the bracts spread for 1 to 2 days, and the fruit setting rate is the highest; by the pollination stage, the female flowers have completely lost their vitality and cannot bear fruit.

[0053] Table 2 The morphological characteristics of konjac bracts and hybrid seed setting rate

[0054]

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Abstract

The invention discloses a method for amorphophallus virosus crossbreeding. The method comprises the following technical steps: cultivating flowering plants, measuring the vitality of pollens, preparing female flower maturity judgment criteria, performing pollination, seed harvesting and preservation, seedling transplanting, and the like. According to the flowering habit of amorphophallus virosus, by the application of technical measures such as reasonable selection of flowering seed taros, measurement of the vitality of the pollens, accurate mastering of the time node of female flower maturity, artificial auxiliary pollination at proper time and scientific seed harvesting and preservation, artificially hybridized seeds can be obtained continuously for many years, and the hybridization success rate is 90 percent or above; the first-generation seed taros have the characteristics of low virus accumulation and high yield. According to the method for amorphophallus virosus crossbreeding, the difficulty of non fruiting of amorphophallus virosus under a natural condition is solved, and a foundation is laid for development and application of high-quality amorphophallus virosus seed resources; a new hope is brought to solve the world problem of soft rot diseases of amorphophallus virosus; the method has the advantages of being simple in operation, low in cost, high in fruiting rate and the like.

Description

Technical field [0001] The invention belongs to the technical field of plant seed cultivation, and specifically relates to a method for cross breeding of Amorphophallus wartii. Background technique [0002] Konjac (Amorphophalluskonjac) belongs to the Amorphophallus (Amorphophallus) plant of the Araceae family. Amorphophallus konjac is an economic crop with high value for edible, medicinal, health care and industrial purposes. The pharmacological effect of konjac is konjac extracted from its tubers. Glucomannan (KGM) is widely used in food processing, daily chemicals, health products, etc. due to its water absorption, gelling, cohesive, low-heat and edible properties. However, konjac has serious disease, low reproduction coefficient of planting taro, and high production risk. Soft rot is a devastating disease in the production of konjac. It is called "konjac cancer". At present, there is no effective control agent, which has long restricted the development of the konjac industry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 徐文果郑宇峰黄廷祥张晓梅岩所张国云张磊车宏志赵庆云陈军文黄炜李佳芹李兴清王艳谢世清
Owner DEHONG DAI JINGPO AUTONOMOUS PREFECTURE AGRI TECH PROMOTION CENT
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