Application of aldehyde dehydrogenases 1A3 and encoding gene thereof as target for preventing and treating invasion and metastasis of colorectal cancer
A technology of acetaldehyde dehydrogenase and colorectal cancer, applied in the field of biomedicine, can solve problems such as unclear molecular regulation mechanism
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[0032] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental methods for which specific conditions are not indicated in the preferred embodiments are usually carried out according to conventional conditions, or according to the conditions suggested by the manufacturer.
[0033] In a preferred embodiment, the coding DNA sequence of the shRNA that inhibits ALDH1A3 gene expression is any one of the following three sequences (the underlined part is the loop sequence):
[0034] 1: gctgtattagaaccctcagatctc cgggatccaa gagatctgagggttctaatacagcttttttg (SEQ ID No. 1);
[0035] 2: gccgaatacacagaagtgaaactc cggatccaa gagtttcacttctgtgtattcggcttttttg (SEQ ID No. 2);
[0036] 3: aaccaatactgaagttcaactc cgggatccaa gagttgaacttcagtattggttgcttttttg (SEQ ID No. 3).
[0037] The construction method of ALDH1A3 gene shRNA expression vector comprises the following steps:
[0038] 1) Synthesize the foll...
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