Lactic acid bacteria for efficiently degrading nitrite and application thereof in pickling salt
A technology of nitrite and lactic acid bacteria is applied in the field of bioengineering to achieve the effect of strong acid-producing ability and high-efficiency degradation
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[0029] Screening of Strains with Strong Acid Production Ability
[0030] The lactic acid bacteria in the naturally fermented kimchi samples were enriched and cultivated by using MRS liquid medium, and the culture solution was serially diluted ten-fold, and added 2.0% (w / v) CaCO 3 The MRS culture medium was used as the separation medium to prepare pouring plates, cultured at 37°C for 48 hours, and the colony with the larger diameter of the transparent circle and the diameter of the colony was selected for isolation and purification. lactic acid. The isolated strains were inoculated into MRS liquid medium, cultured at 37°C for 48 hours, and the total acid in the fermentation liquid of each isolated strain was determined by potentiometric titration. figure 1 . Three strains with strong acid production ability were obtained, namely A1, A2 and A3.
[0031] Screening of Lactic Acid Bacteria Strains with Efficient Degradation of Nitrite
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