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Lactic acid bacteria for efficiently degrading nitrite and application thereof in pickling salt

A technology of nitrite and lactic acid bacteria is applied in the field of bioengineering to achieve the effect of strong acid-producing ability and high-efficiency degradation

Active Publication Date: 2016-06-15
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, lactic acid bacteria with strong acid-producing ability and high-efficiency degradation of nitrite are prepared by spray-drying into a viable bacterial agent, and added to edible salt to prepare pickle salt. This technology and product have not been reported yet.

Method used

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  • Lactic acid bacteria for efficiently degrading nitrite and application thereof in pickling salt
  • Lactic acid bacteria for efficiently degrading nitrite and application thereof in pickling salt
  • Lactic acid bacteria for efficiently degrading nitrite and application thereof in pickling salt

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Embodiment 1

[0029] Screening of Strains with Strong Acid Production Ability

[0030] The lactic acid bacteria in the naturally fermented kimchi samples were enriched and cultivated by using MRS liquid medium, and the culture solution was serially diluted ten-fold, and added 2.0% (w / v) CaCO 3 The MRS culture medium was used as the separation medium to prepare pouring plates, cultured at 37°C for 48 hours, and the colony with the larger diameter of the transparent circle and the diameter of the colony was selected for isolation and purification. lactic acid. The isolated strains were inoculated into MRS liquid medium, cultured at 37°C for 48 hours, and the total acid in the fermentation liquid of each isolated strain was determined by potentiometric titration. figure 1 . Three strains with strong acid production ability were obtained, namely A1, A2 and A3.

[0031] Screening of Lactic Acid Bacteria Strains with Efficient Degradation of Nitrite

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Abstract

The invention belongs to the field of biotechnology, and particularly discloses lactic acid bacteria for efficiently degrading nitrite and application thereof in pickling salt.The classification of the lactic acid bacteria is named as Lactobacillus plantarum, the strain of the Lactobacillus plantarum is preserved in China Center for Type Culture Collection with the preservation number being CCTCC NO: M 2015184, the acid production speed of the stain of the Lactobacillus plantarum in 48 h is 3.0*10<-2>-4.0*10<-2> g / 100 mL.h, and the nitride degradation rate is 88.0-98.0%.The lactic acid bacteria strain obtained through screening is strong in acid production capacity and capable of efficiently degrading nitride, is prepared into a live bacteria agent through spray drying and added into salt to be prepared into pickling salt used for pickled vegetable pickling, has the advantages that lactic acid fermentation is rapid and nitrite degradation ability is high, and the pickled vegetable eating safety can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a lactic acid bacterium for efficiently degrading nitrite and its application in pickle salt. Background technique [0002] The history of my country's folk pickled pickles is very long, and many places have the traditional consumption habit of producing and eating by themselves. Domestic pickled kimchi adopts natural fermentation, that is, after the vegetables are washed and dried, they are fermented and pickled with edible salt water with a NaCl concentration of about 8% (w / v). During this period, the lactic acid bacteria attached to the vegetable ferment to produce lactic acid, and a large number of other microorganisms including some harmful bacteria participate in it. However, as the acid production of lactic acid bacteria increases, the pH value of the kimchi brine decreases, and the growth of miscellaneous bacteria will be inhibited. However, if only the lactic aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L27/40A23L29/00C12R1/25
CPCC12N1/20A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2250/1614A23V2250/636
Inventor 黄丹刘有晴罗惠波于华毛祥叶光斌
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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