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SNP site related to growth trait of paralichthys olivaceus as well as screening method and application of SNP site

A technology of growth traits and screening methods, applied in the field of screening in aquaculture, can solve the problems of long breeding cycle, low efficiency, lack of effective breeding methods for target traits, etc., and achieve the effect of increasing selection intensity and shortening breeding cycle

Active Publication Date: 2016-06-01
中国水产科学研究院北戴河中心实验站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Conventional breeding is based on the phenotype of the trait instead of the genotype. There is no effective breeding method for the target trait, and the breeding cycle is long and the efficiency is low.
So far, there is no report on SNP markers of flounder GH gene related to growth traits

Method used

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  • SNP site related to growth trait of paralichthys olivaceus as well as screening method and application of SNP site
  • SNP site related to growth trait of paralichthys olivaceus as well as screening method and application of SNP site
  • SNP site related to growth trait of paralichthys olivaceus as well as screening method and application of SNP site

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A SNP site related to the growth traits of flounder, the SNP site is the 33rd bp position of the fourth intron in the GH gene whose sequence is SEQ ID NO: 1, and its base is G or A.

Embodiment 2

[0032] A method for screening SNP sites described in Example 1, the sequence of the upstream primer used is shown in SEQ ID NO: 2; the sequence of the downstream primer is shown in SEQ ID NO: 3.

[0033] Wherein, the sequence of SEQ ID NO: 2 is AGGTTACATCCAAACTGTCAG;

[0034] The sequence of SEQ ID NO: 3 is GTGAGCTGGAGCACCGAACTC.

[0035] In the process of amplifying the GH gene using the upstream and downstream primers, the reaction system used is: total volume 50 μL, DNA 1 μL, 10*buffer 5 μL, Mg 2+5 μL, dNTP 1 μL, primer 1 μL / strip, Taq enzyme 0.6 μL, the balance is water.

[0036] The PCR amplification program used was: pre-denaturation at 94°C for 3 min; then 35 cycles, each cycle including the following steps in turn: denaturation at 94°C for 15 s, annealing at 55°C for 15 s, Extend at 72°C for 30s; after completing 35 cycles, extend at 72°C for 3min.

Embodiment 3

[0038] A method for screening flounder with the SNP site described in Example 1, comprising the following steps:

[0039] A. Screening of flounder: according to the growth traits index in GB / 18654.3-2008 as a standard, different flounders are screened, and the growth traits indexes include but not limited to full length, body length and body height; after the flounder is screened out, the Each flounder was induced to obtain different gynogenetic families and / or heterozygous clonal families, and each gynogenetic family was marked; in this embodiment, 3 different gynogenetic families were selected, and 3 different gynogenetic families were selected. 6 different heterozygous clonal families, fluorescently labeled 6 different families;

[0040] B, polyculture in the same pond: the flounder after marking is polycultured in the same pond for 15 months;

[0041] C. Genotyping: After 15 months of breeding, each family randomly selected 20 tails, a total of 120 tails, and identified t...

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Abstract

The invention relates to an SNP site related to growth trait of paralichthys olivaceus as well as a screening method and application of the SNP site. Correlation analysis of SNP in GH genes and the growth trait of paralichthys olivaceus is performed by the candidate gene correlation analysis method to obtain an SNP marker related to growth trait, and the SNP marker can be used for marker-assisted selection of paralichthys olivaceus to shorten the breeding cycle.

Description

technical field [0001] The invention belongs to the technical field of screening in aquaculture, and in particular relates to a SNP site related to the growth traits of flounder, a screening method and application thereof. Background technique [0002] Flounder (Paralichthysolivaceus), also known as partial mouth, left mouth, left partial, tooth piece, etc., belongs to the Pleuronectiformes (Pleuronectiformes), family Bothidae, flounder genus, mainly distributed in the Far East of Russia, Japan, South Korea and my country's coastal areas belong to the indigenous species. It is large, fast-growing and delicious, and is favored by East Asian countries. [0003] Fish growth hormone (GH) is a single-chain polypeptide hormone with a wide range of physiological effects, which can promote protein synthesis in muscles and accelerate the longitudinal growth of fish bones. It plays an important role in sexual maturity and enhancing the adaptability of fish to high-salt environment. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 侯吉伦王桂兴张晓彦王玉芬刘海金于清海杨立更
Owner 中国水产科学研究院北戴河中心实验站
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