Bacterial magnetic particles with recombinant protein G expression capability and application thereof
A technology of bacterial magnetic particles and recombinant proteins, which is applied in the application of nano magnetic beads and medical testing, can solve the problems of increasing the cost of anti-human ball tests, and achieve the effects of improving stability, improving amplification ability, and enhancing load capacity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0048] Example 1 Bacterial magnetic particles expressing recombinant protein G
[0049] A bacterial magnetic particle expressing recombinant protein G, the bacterial magnetic particle expressing recombinant protein G is obtained by expanding, culturing, separating and purifying the recombinant strain of magnetotactic bacteria MSR-1.
Embodiment 2
[0050] Example 2 Bacterial magnetic particles expressing recombinant protein G
[0051] A bacterial magnetic particle expressing recombinant protein G, the bacterial magnetic particle expressing recombinant protein G is obtained by expanding, culturing, isolating and purifying a recombinant strain of magnetotactic bacteria MSR-1;
[0052] The sequence of the recombinant protein G is:
[0053] The magnetotactic bacteria MSR-1 recombinant strain is constructed by the following method:
[0054] a. Construction of a MSR-1 mutant strain of magnetotactic bacteria with deletion of the bacterial magnetic particle membrane protein mamC / mamF gene;
[0055] b. Construction of bacterial magnetic particle membrane protein fusion expression plasmid;
[0056] c. Transfer the functionalized bacterial magnetic particle fusion expression plasmid into the magnetotactic bacteria MSR-1 mutant strain obtained in step a by conjugation to construct the magnetotactic bacteria MSR-1 recombinant s...
Embodiment 3
[0057] Example 3 Bacterial magnetic particles expressing recombinant protein G
[0058] A bacterial magnetic particle expressing recombinant protein G, the bacterial magnetic particle expressing recombinant protein G is obtained by expanding, culturing, isolating and purifying a recombinant strain of magnetotactic bacteria MSR-1;
[0059] The sequence of the recombinant protein G is:
[0060] The preparation method of the bacterial magnetic particles expressing recombinant protein G is as follows:
[0061] a. Construction of a MSR-1 mutant strain of magnetotactic bacteria with deletion of the bacterial magnetic particle membrane protein mamC / mamF gene;
[0062] (a) Amplification of bacterial magnetic particle membrane protein mamC / mamF gene with two homologous fragments on the left and right sides of 700-1000 bp in length; both left and right homologous fragments have two restriction sites;
[0063] (b) The left and right homologous fragments are respectively double dige...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com