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Methods for classifying pleural fluid

A technology for pleural fluid and exudate, which is used in biochemical equipment and methods, pharmaceutical formulations, emulsion delivery, etc.

Inactive Publication Date: 2016-05-04
THE UNIVERSITY OF HONG KONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, none of these assays can achieve both sensitivity and specificity greater than 90%
Currently, no single marker has satisfactory sensitivity and specificity for both

Method used

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  • Methods for classifying pleural fluid
  • Methods for classifying pleural fluid
  • Methods for classifying pleural fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0105] Pleural effusion NMR signals of lipid particles as biomarkers of exudative pleural effusions

[0106] Materials and methods

[0107] Sample Preparation

[0108] Samples were collected into sterile regular bottles, followed by centrifugation at 3000 rpm for 10 minutes at 4°C to obtain a clear supernatant. At the time of analysis, mix 480 μL sample with 120 μL buffer solution (in 100% D 2 1.5MKH in O 2 PO 4 )mix. Samples were pooled and 550 μL of each were freshly transferred to 5 mm NMR tubes for NMR analysis.

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Abstract

Methods of classifying pleural fluid are disclosed. The methods typically include determining the level of indicator nanoparticles, such as lipids, particularly large lipids, in the pleural fluid of a subject. The level of lipids can be determined by nuclear magnetic resonance (NMR), such as proton NMR (1H-NMR) by measuring the NMR signal corresponding to methyl protons, methylene protons, methene protons, or combinations thereof. The level of large lipids in pleural fluid can be carried out in vitro on a sample of pleural fluid obtained from the subject or in vivo using magnetic resonance spectroscopy (MRS). The pleural fluid can be classified as exudate or transudate with a sensitivity, selectivity, or combination thereof of 85%, 90%, 95%, 99%, or high, a selectivity of 85%, 90%, 95% and 99%. The method can be coupled with diagnosing and / or treating the subject with a disease, disorder, or condition.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application No. 61 / 847610, filed July 18, 2013. Application No. 61 / 847610, filed July 18, 2013, is hereby incorporated by reference in its entirety. field of invention [0003] The present invention generally relates to methods of classifying pleural fluid samples as transudates or exudates and the use of these methods to aid in the diagnosis of disease. Background of the invention [0004] Pleural fluid (PF) is the thin liquid layer of the pleural cavity that serves as an important physiological lubricant and mechanical barrier. The volume and flow of PF are tightly regulated. In disease states, the dynamics of PF flow are impaired, leading to abnormal accumulation of PF. This condition is called pleural effusion. [0005] In clinical practice, the first step in investigating the etiology of pleural effusions is generally to classify PF as either exudative (hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/14
CPCA61B5/055A61B5/08G01R33/465A61B5/14507A61B5/14532A61B5/14539A61B5/14546A61B5/1455A61B5/4839C12Q1/32G01N33/92G01R33/44
Inventor 林青云
Owner THE UNIVERSITY OF HONG KONG
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