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Lung cancer screening test kit

A technology of kits and reagents, applied in the field of lung cancer screening kits, to achieve good results in clinical application prospects

Active Publication Date: 2016-05-04
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the relationship between brain-specific inhibitor of neovascularization factor 1 and lung cancer

Method used

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  • Lung cancer screening test kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1BAI-1

[0022] Example 1 Relationship between BAI-1 expression level and lung cancer

[0023] 1. Experimental method

[0024] 1. Clinical data

[0025] 60 lung cancer patients and 34 healthy controls were selected. The basic information is as follows:

[0026]

[0027] * Note: Others include large cell carcinoma, adenosquamous carcinoma, and small cell carcinoma.

[0028] 2. Detection of BAI-1 expression level (quantitative detection of BAI-1 content)

[0029] The blood of lung cancer patients and healthy controls was extracted, centrifuged after EDTA anticoagulation (1200rpm, 10min), the supernatant was sucked and then centrifuged for the second time (3000rpm, 15min), and the supernatant after the second centrifugation was taken as the test specimen. Store at -80°C after aliquoting.

[0030] Using the BAI-1 ELISA kit purchased from BIOMATIK (Cat. No.: EKU02798), the expression level of BAI-1 was detected according to the following method:

[0031] (1) Add 100 μl / well of stan...

Embodiment 2

[0049] Embodiment 2 Composition and method of use of the kit for detecting the expression level of BAI-1 in blood according to the present invention

[0050] 1. ELISA detection kit

[0051] 1. The composition of the kit

[0052] Detection kit (50 copies):

[0053] components

volume

BAI-1 Antibody Coated Plate

1

BAI-1 standard

1ml (20000pg / ml)

Detection reagent A

120ul

Detection reagent B

120ul

Detection Reagent A Diluent

12ml

Detection Reagent B Diluent

12ml

TMB substrate reaction solution

9ml

detergent

20ml

stop solution

6ml

[0054] 2. How to use the kit

[0055] Draw the blood of the sample to be tested, centrifuge (1200rpm, 10min) after EDTA anticoagulation, absorb the supernatant and perform a second centrifugation (3000rpm, 15min), absorb the supernatant after the second centrifugation, and use it as a test sample, according to the following method T...

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Abstract

The invention discloses a lung cancer screening test kit. The lung cancer screening test kit comprises an optional reagent used for testing the expression level of BAI-1. The invention further discloses the application of the reagent used for testing the expression level of BAI-1 in preparing a lung cancer screening test reagent. By testing the expression level of BAI-1, the risk of being affected with lung cancer of people to be tested can be judged. The kit can be used for auxiliary diagnosis of clinical lung cancer, an effective basis is provided for taking corresponding treatment measures or making decisions on patients, and clinical application prospects are broad.

Description

technical field [0001] The invention relates to a lung cancer screening kit. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] The etiology of lung cancer is complex, and it is generally believed that the influencing factors include: ① smoking; ② environmental pollution: such as smog, interior decoration; ③ unhealthy lifestyle: such as poor eating habits, high life pressure; Silicosis, chronic bronchitis; ⑤ Intrinsic factors in the human body: such as family inheritance, decreased immune function, endocrine dysfunction, etc. [0004] At the same time, lung cancer is a disease that is good at hiding. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57423G01N33/57488
Inventor 张立李为民刘伦旭刘丹王业唐怀蓉黄燕田攀文朱苡晗谭政堂王彦文
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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