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Antibacterial culture medium for tissue culture of plants and preparation method of culture medium

A plant tissue culture and culture medium technology, applied in the field of plant tissue culture antibacterial medium and its preparation, can solve the problems of time-consuming, labor-intensive, and inability to effectively reduce the pollution rate of tissue culture seedlings, so as to reduce the pollution rate and improve rooting rate, increase the effect of germination rate

Inactive Publication Date: 2016-05-04
龙岩市禾康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is not only time-consuming and labor-intensive, but also cannot effectively reduce the contamination rate of tissue culture seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of primary culture medium: add 20g sucrose and 8g agar to 1L distilled water and stir to sterilize. After sterilization, cool it down to about 55°C. Ling, 200mg urotropine, 600mg propamocarb hydrochloride were added and mixed well; under sterile conditions, 3gMS+6-BA, 600mgNAA, 500mgIAA, 100mg zinc chloride, 500mg vitamin B 1 , 600mg vitamin B 6 Add and mix thoroughly to prepare the primary culture medium.

[0021] Proliferation and subculture medium preparation: add 20g sucrose and 8g agar to 1L distilled water and stir to sterilize it. After sterilization, cool it down to about 55°C. Under sterile conditions, add 2g carboxylated chitosan, 20mg antibiotic, Add hymexazol, 200mg urotropine, 600mg propamocarb hydrochloride and mix well; under sterile conditions, add 800mgMS+6-BA, 500mgNAA, 3gCCC, 400mg vitamin B 1 , 400mg Vitamin B 2 , 200mg vitamin B 6 , 100mg Vitamin B 9 Add and mix well;

[0022] Select robust plant tissue explants, inoculate the expl...

Embodiment 2

[0025] Preparation of the primary culture medium: add 25g sucrose and 9g agar to 1L distilled water and stir to sterilize it. After sterilization, cool it down to about 55°C. Ling, 300mg urotropine, 700mg propamocarb hydrochloride were added and mixed well; under sterile conditions, 3.5gMS+6-BA, 700mgNAA, 600mgIAA, 150mg zinc chloride, 750mg vitamin B 1 , 700mg vitamin B 6 Add and mix thoroughly to prepare the primary culture medium.

[0026] Proliferation and subculture medium preparation: add 25g sucrose and 9g agar to 1L distilled water and stir to sterilize it. After sterilization, cool it down to about 55°C. Under sterile conditions, add 3g carboxylated chitosan, 30mg antibiotic, Add hymexazol, 300mg urotropine, 700mg propamocarb hydrochloride and mix well; under sterile conditions, add 900mgMS+6-BA, 600mgNAA, 3.5gCCC, 500mg vitamin B 1 , 500mg Vitamin B 2 , 300mg Vitamin B 6 , 150mg Vitamin B 9 Add and mix well;

[0027] Select robust plant tissue explants, inocula...

Embodiment 3

[0030] Preparation of primary culture medium: add 30g sucrose and 10g agar to 1L distilled water and stir to sterilize. After sterilization, cool it down to about 55°C. Ling, 400mg urotropine, 800mg propamocarb hydrochloride were added and mixed well; under sterile conditions, 4gMS+6-BA, 800mgNAA, 700mgIAA, 200mg zinc chloride, 1000mg vitamin B 1 , 800mg Vitamin B 6 Add and mix thoroughly to prepare the primary culture medium.

[0031] Proliferation subculture medium configuration: add 30g sucrose and 10g agar to 1L distilled water and stir to sterilize, after sterilization, cool it down to about 55°C. Under sterile conditions, add 4g carboxylated chitosan, 40mg antibiotic, Add hymexazol, 400mg urotropine, 800mg propamocarb hydrochloride and mix well; under sterile conditions, add 1000mgMS+6-BA, 700mgNAA, 4gCCC, 600mg vitamin B 1 , 600mg vitamin B 2 , 400mg Vitamin B 6 , 200mg vitamin B 9 Add and mix well;

[0032] Select robust plant tissue explants, inoculate the explant...

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PUM

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Abstract

The invention discloses an antibacterial culture medium for tissue culture of plants and a preparation method of the culture medium. A primary culture medium contains carboxylation chitosan, an antibiotic, hymexazol, urotropin, propamocarb hydrochloride, MS+6-BA(6-benzyladenine), naphthalene acetic acid, auxin and the like; a subculture propagation culture medium contains carboxylation chitosan, an antibiotic, hymexazol, urotropin, propamocarb hydrochloride, MS+6-BA, naphthalene acetic acid, chlormequat chloride and the like. Antibacterial agents are added to the primary culture medium and the subculture propagation culture medium, the pollution rate of the plants in the primary tissue culture and tissue subculture processes is reduced, the germination rate and the rooting rate of the plants during tissue culture are increased, and the economic benefits are improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a plant tissue culture antibacterial medium and a preparation method thereof. Background technique [0002] Plant tissue culture technology has been widely used in the propagation of plant seedlings as a rapid plant propagation technology. In the process of plant tissue culture, the key link is the contamination rate of plant tissue culture, followed by the germination rate and rooting rate in the tissue culture process. For a long time, in order to reduce the contamination rate in the process of plant tissue culture, the explants are usually sterilized to control the contamination rate by increasing the strictness of the manual operation. However, this method is not only time-consuming and labor-intensive, but also cannot effectively reduce the contamination rate of tissue culture seedlings. Therefore, establishing an effective antibacterial medium for plant tissu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张玉华樊仲书王海斌樊昌华孔祥海樊君早陈晓婷
Owner 龙岩市禾康生物科技有限公司
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