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Light-matrix degradable-nonwoven-fabric ex-vitro-rooting seedling raising method for Hosta plantaginea tissue culture seedlings

A technology of tissue culture seedlings and light substrate, which is applied in the field of plant tissue culture, can solve the problems of large environmental damage and source limitation, achieve good ventilation, reduce production costs, and save tissue culture space

Inactive Publication Date: 2016-05-04
SICHUAN AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, peat, the main material in the matrix, is a product of natural swamps formed over thousands of years, rich in nutrients, but peat is a non-renewable resource, and large-scale mining will cause great damage to the environment; although the use of straw pulp and papermaking waste is waste utilization, its source To varying degrees limited by the location of production

Method used

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  • Light-matrix degradable-nonwoven-fabric ex-vitro-rooting seedling raising method for Hosta plantaginea tissue culture seedlings
  • Light-matrix degradable-nonwoven-fabric ex-vitro-rooting seedling raising method for Hosta plantaginea tissue culture seedlings
  • Light-matrix degradable-nonwoven-fabric ex-vitro-rooting seedling raising method for Hosta plantaginea tissue culture seedlings

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Experimental program
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Effect test

Embodiment 1

[0024] Specifically include the following steps:

[0025] 1) Substrate preparation: Select perlite, vermiculite, coconut peat and plant ash as the content of the matrix, mix them evenly, and configure them into No. Plant ash, perlite and vermiculite; No. 2 substrate is plant ash, perlite, vermiculite and coconut peat with a volume ratio of 1:1:1:1; fill the two substrates until the diameter of the substrate is 5cm and the height is 7cm non-woven bag, neatly placed on the tray, soaked with 1000 times chlorothalonil and 1000 times carbendazim mixture for disinfection, and sprayed again before official use to enhance the disinfection effect.

[0026] 2) Transition seedling hardening: after tissue culture and multiplication, transfer the clustered seedlings of Hosta japonica from the tissue culture room to airtight hardening under natural light for 2-3 days, then loosen the lid for 1-2 days, and then completely open the lid for 2 days , let the bottle seedlings gradually adapt to...

Embodiment 2

[0035] The method of rooting seedlings outside hosta mosaic tissue culture seedlings with degradable non-woven bag and bottle with light substrate, specifically includes the following steps:

[0036] 1) Transplanting substrate preparation: choose perlite, vermiculite, coconut peat and plant ash as the substrate content, mix them evenly, and configure No. 1 substrate and No. 2 substrate. Among them, No. 1 substrate is: the volume ratio is 1:1 : 1 plant ash, perlite and vermiculite; No. 2 matrix is ​​plant ash, perlite, vermiculite and coconut peat with a volume ratio of 1:1:1:1; fill the two matrixes until the diameter of the matrix is ​​5cm , in a non-woven bag with a height of 7cm, neatly placed on a tray, soak the substrate with a mixture of 1000 times chlorothalonil and 1000 times carbendazim for disinfection, and spray again before official use to enhance the disinfection effect.

[0037] 2) Transition seedling hardening: Transfer the hosta vase seedlings after tissue cult...

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Abstract

The invention discloses a light-matrix degradable-nonwoven-fabric ex-vitro-rooting seedling raising method for Hosta plantaginea tissue culture seedlings. The method specifically comprises the following steps of carrying out matrix preparation, carrying out transition seedling hardening, carrying out seedling selecting and plant division, carrying out rooting agent preparing and rooting agent treatment, carrying out tissue culture seedling transplanting and carrying out transplanted management. According to the method, the production cost can be reduced by 27%, and the tissue culture space is saved by 35%, so that the method is of great practical significance in ecological environmental protection and the industrialized production of excellent Hosta plantaginea varieties.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rooting seedlings outside a bottle of hosta tissue culture seedlings with degradable light matrix and non-woven fabrics. Background technique [0002] Hosta (Hosta) is a perennial herb of Liliaceae (Liliaceae), native to the East, and the wild species are distributed most in Japan, followed by China. Due to its rich variety, unique shape, long viewing period and strong adaptability, it can be used as an ideal ground cover for foliage and flower viewing, understory plants, potted ornamental plants and cut flowers. In addition, because it is rich in polysaccharides, saponins, trace elements and other components, it has high medicinal value and is one of the medicinal plants with great development prospects in the future. It has become one of the top selling herbal flowers in the world and is widely cultivated all over the world. [0003] Purp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G24/00A01G31/00
Inventor 谢程程陈友祥李西蔡仕珍孙凌霞熊英
Owner SICHUAN AGRI UNIV
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