Method for adjusting and controlling nicotine content of tobacco
A nicotine content technology, applied in botany equipment and methods, horticulture, application, etc., to achieve high grafting survival rate, low nicotine content, and good affinity
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Embodiment 1
[0026] The acquisition of embodiment 1 grafted seedling
[0027] (1) Rootstock cultivation
[0028] On March 9th, the seeds of Torubum were soaked in a gibberellin solution with a concentration of 100mg / L for 48h, washed, and put into a climate box on March 11th for variable temperature germination (25°C during the day, 12h; 16°C for 12 hours), and on March 17, 50% of the seeds turned white, and they were sown into the substrate (flue-cured tobacco seedling cultivation substrate) by the tray seedling raising method, 1 seed per hole. After sowing, the seedling trays were placed in the greenhouse, and water and fertilizer management was strengthened.
[0029] (2) Scion cultivation
[0030] On March 23, when more than 50% of the rootstock seedlings had two cotyledons unfolded, flue-cured tobacco seedling cultivation was carried out, the variety was K326 (coated seeds), and the floating seedling cultivation method was adopted, and the substrate was selected as a commercially ava...
Embodiment 2
[0033] The field test of embodiment 2 grafted seedlings
[0034] On May 20th, the flue-cured tobacco grafted seedlings were transplanted, and the grafted seedlings and K326 self-rooted seedlings were transplanted to the field with a row spacing of 0.5m*1.2m, and the grafting joint was about 2cm above the ridge surface. Transplant the grafted seedlings to the field. When transplanting, be careful not to plant the grafted wound into the soil. When the soil is uncovered 30 days after transplanting, the induced amount of adventitious roots can be artificially controlled by setting different heights of the soil to achieve control of nicotine synthesis. Quantitative purpose.
[0035] There are 4 treatments in the experiment, and the specific treatment methods are as follows:
[0036] Table 1 Detailed table of soil height treatment
[0037] deal with
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The grafted seedlings are as high as 70-80cm, and the film is removed and the soil is...
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