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Detection method for peripheral blood circulating tumor cell VEGF of patient suffering from advanced colorectal carcinoma

A technology for peripheral blood circulation and colorectal cancer, applied in the field of detection of VEGF, which can solve problems such as difficulty in evaluating the expression status of VEGF

Inactive Publication Date: 2016-04-20
SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT
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Problems solved by technology

For patients with advanced colorectal cancer whose tissue samples cannot be obtained, the expression status of VEGF becomes difficult to assess

Method used

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  • Detection method for peripheral blood circulating tumor cell VEGF of patient suffering from advanced colorectal carcinoma
  • Detection method for peripheral blood circulating tumor cell VEGF of patient suffering from advanced colorectal carcinoma
  • Detection method for peripheral blood circulating tumor cell VEGF of patient suffering from advanced colorectal carcinoma

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Embodiment Construction

[0039] Below in conjunction with accompanying drawing and embodiment the present invention is described as follows.

[0040] An example of using this technical method to separate, obtain and identify peripheral blood circulating tumor cells of 8 patients with advanced colorectal cancer (while detecting 8 samples of normal people as negative controls).

[0041] 1. Using a membrane filtration device to separate and obtain CTCs in the peripheral blood of patients with advanced colorectal cancer:

[0042]Collect 5ml of peripheral blood from each sample, dilute the peripheral blood with 45ml diluent (composition: 1mmol / lEDTA+0.1%BSA), and then add 3ml of 4% paraformaldehyde to fix the diluted blood sample for 10 minutes.

[0043] At fixed intervals, assemble the membrane filtration device: as attached figure 1 , figure 2 , image 3 As shown, the filter device is composed of a filter 3 , a filter membrane 7 , a blood sample container 2 , a waste liquid tank 5 , and an iron stand...

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Abstract

The invention discloses a detection method for the peripheral blood circulating tumor cell VEGF of a patient suffering from advanced colorectal carcinoma. CTC in peripheral blood of the patient suffering from advanced colorectal carcinoma is obtained by conducting separation through a membrane filtration device; a thin cell layer is manufactured through the cell paraffin block technology; the VEGF gene expression situation of the patient suffering from colorectal carcinoma is detected. By means of the technical method, the VEGF expression situation of the patient where tissue samples can not be obtained can be detected without using colorectal carcinoma tissue. The technology is minimally invasive, and real-time detection can be conducted.

Description

technical field [0001] The invention relates to a new method for detecting VEGF, especially a method for circulating tumor cell VEGF in peripheral blood of patients with advanced colorectal cancer. Background technique [0002] For patients with advanced colorectal cancer, targeted combination chemotherapy can significantly prolong the disease-free progression-free survival and overall survival of advanced colorectal cancer. Bevacizumab, a vascular endothelial growth factor (VEGF) receptor inhibitor, has achieved remarkable curative effect in clinical practice. [0003] VEGF is a homodimeric glycoprotein produced by both normal and tumor cells and plays an important role in angiogenesis. The expression level of VEGF was significantly higher in tumor tissues than in non-tumor tissues. VEGF is the most important pro-angiogenic factor discovered so far. It can promote the proliferation and migration of vascular endothelial cells and prevent the apoptosis of vascular endotheli...

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57419G01N33/68G01N2333/475
Inventor 王爱亮薛雁李胜王振丹
Owner SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT
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