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A preparation method of algae suspended and stable samples for fluorescence measurement

A fluorescence measurement and suspension stability technology, which is applied in the preparation of test samples, measurement devices, fluorescence/phosphorescence, etc., can solve the problems of inaccurate measurement results, unstable suspension system, uneven algae cells, etc., and achieve suspension stability High, stable algae liquid system, accurate measurement effect

Active Publication Date: 2018-06-19
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0004] The present invention provides a method for solving the problems of inaccurate measurement results and large errors due to the unevenness of algae cells in the water body, easy sedimentation and unstable suspension system when measuring the chlorophyll content of water samples by fluorescence method in the prior art. The preparation method of algae suspended and stable samples for fluorescence measurement effectively solves the above problems

Method used

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  • A preparation method of algae suspended and stable samples for fluorescence measurement
  • A preparation method of algae suspended and stable samples for fluorescence measurement
  • A preparation method of algae suspended and stable samples for fluorescence measurement

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Embodiment 1

[0020] Embodiment 1. take Chlorella pyrenoidosa as test algae

[0021] The selected Chlorella pyrenoidosa (purchased from the Wild Biomass Bank of the Chinese Academy of Sciences—Freshwater Algae Species Bank) was used as the algae species, and the algae species were inoculated in the Erlenmeyer flask filled with sterilized BG-11 culture medium, and then the conical The shaped bottle was cultured in an E-30B0 light incubator. The culture conditions were: culture temperature 25°C, light condition 2000lx, time set to 12h day / 12h night, humidity condition 75%RH, algae cultured statically, shaking every day Change the position of the Erlenmeyer flask twice at the same time, and take it out at the same time after ten days as the algae liquid for testing;

[0022] Take 25mL of the algae liquid to be tested and place it in a centrifuge, centrifuge at a speed of 4000r / min for 15min, then pour off the supernatant to obtain the algae, and then prepare the obtained algae with a suspensio...

Embodiment 2

[0059] Embodiment 2. Actual water sample is for testing algae

[0060] Collect 4L of artificial lake water from Hebei University of Science and Technology as the actual water sample, divide it into 4 parts on average, get water sample 1, water sample 2, water sample 3, water sample 4, put them in a centrifuge for centrifugation, pour off the supernatant to get algae Algae body, prepare algae body, suspension stabilizer, and water, set the volume to 100mL, stir well, so that the algae cells are evenly distributed in the suspension liquid, and obtain the algae liquid sample. Use the RF5301 fluorescence spectrophotometer to measure the fluorescence intensity value of the algae liquid sample, reflect the algae content in the water body through its fluorescence value intensity, and reflect the suspension of the algae liquid sample solution according to the change rate of the fluorescence intensity value and the relative standard deviation of the measured value stability.

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Abstract

The invention discloses a preparation method of an algae suspension stable sample for fluorescent measurement, and belongs to the technical field of algae liquid measurement. The method includes the steps of preparing an algae liquid sample through to-be-sampled algae liquid, measuring chlorophyll in the algae liquid sample through a fluorescent method, conducting centrifuging treatment on the to-be-sampled algae liquid, and preparing an algae liquid sample through algae obtained through centrifuging treatment and a suspension stabilizer, wherein the suspension stabilizer is prepared from agar, xanthan gum and carrageenan. By adding the suspension stabilizer to the detected algae liquid in the fluorescent detecting process, all parameters are measured through florescence, the fluorescence value is small in attenuation rate and fluctuation, it is shown that the algae is high in suspension stability and stable in system, and the algae liquid measurement is more accurate.

Description

technical field [0001] The invention belongs to the technical field of measuring chlorophyll content of water samples, and relates to a method for measuring chlorophyll content of water samples by a fluorescence method, more specifically, a method for preparing a suspended and stable sample of algal bodies for fluorescence measurement. The suspension and stability of the liquid can be obtained to obtain a uniformly dispersed algae liquid sample. Background technique [0002] Chlorophyll-a content in water is a routine monitoring item in water quality environment monitoring and one of the indicators of water eutrophication. It reflects the number of algae and water quality in water to a certain extent. Accurate determination of chlorophyll a content is particularly important. Due to its high sensitivity, simple operation, continuous real-time monitoring, and the realization of real-time in situ analysis of chlorophyll in water, fluorescence spectrophotometry is the most comm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N1/28
CPCG01N1/28G01N21/6486
Inventor 崔建升樊琳琳段莉丽孙福江
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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