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Liquid suspension culture medium for taxus chinensis cells

A technology of yew cells and medium, which is applied in the field of cell culture and can solve the problems of low yew cell culture density

Inactive Publication Date: 2016-03-23
天津市博爱生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a liquid suspension medium for yew cells, so as to solve the technical problem of the low density of plant cell culture medium for yew cells in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A liquid suspension medium for yew cells, which consists of the following ingredients in parts by weight: 8 parts of sodium nitrate, 4 parts of dipotassium hydrogen phosphate, 2 parts of magnesium sulfate, 1 part of calcium chloride, 1 part of peptone, ascites 1 part, 1 part Duyiwei, 1 part Gushanlong, 1 part Guanglingxiang, 1 part Chinese peach leaf, 50 parts water.

Embodiment 2

[0020] A liquid suspension medium for yew cells, which consists of the following ingredients in parts by weight: 10 parts of sodium nitrate, 6 parts of dipotassium hydrogen phosphate, 3 parts of magnesium sulfate, 2 parts of calcium chloride, 2 parts of peptone, ascites 2 parts, 2 parts of Duyiwei, 2 parts of Gushanlong, 2 parts of Guanglingxiang, 2 parts of Chinese peach leaves, 60 parts of water.

Embodiment 3

[0022] A liquid suspension medium for yew cells, which consists of the following components by weight: 9 parts of sodium nitrate, 5 parts of dipotassium hydrogen phosphate, 2.3 parts of magnesium sulfate, 1.8 parts of calcium chloride, 1.5 parts of peptone, ascites 1.6 parts, 1.7 parts of Duyiwei, 1.8 parts of Gushanlong, 1.2 parts of Guanglingxiang, 1.8 parts of Chinese peach leaves, and 55 parts of water.

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PUM

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Abstract

The invention provides a liquid suspension culture medium for taxus chinensis cells. According to the culture medium, sodium nitrate and dipotassium phosphate serve as a nitrogen source and a phosphorus source respectively, certain calcium chloride is added, and on the basis of the calcium chloride, it is accidentally found that it is helpful to increase cell culture density by adding a certain type of traditional Chinese medicine material of certain ingredients; based on the mentioned beneficial finding, traditional Chinese medicine ingredients such as veronicastrum herbs, lamiophlomis rotata, arcangelisia loureiri, lysimachia foenum-graecum hance, schefflera kwangsiensis, rhizoma anemarrhenae, pericarpium citri reticulatae, kaki calyx and semen allii tuberosi which are originally used for human disease treatment are selected preferentially through experiment means, the obtained culture medium accidentally harvests a remarkable culture effect, the culture density of the taxus chinensis cells is remarkably increased, and meanwhile the growth speed is higher; according to the liquid suspension culture medium, the optimal technical effect is obtained through an innovative technical means, and meanwhile the liquid suspension culture medium is low in cost and easy to implement and thus has good application prospect.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a liquid suspension medium for yew cells. Background technique [0002] Yew, also known as yew, also known as Chinese yew, is a national first-class rare and protected tree species in China, and is also recognized as an endangered natural rare anti-cancer plant in the world. [0003] Paclitaxel and its analogues only exist in the genus Taxus and Australian Taxus in the gymnosperm Taxaceae. There are 11 species of Taxus in the world, which are widely distributed in the frigid, temperate and subtropical regions of Eurasia and North America. area, but is scattered and slow-growing. Taxus genus Australia is only distributed in the southern hemisphere, and its quantity is rare. The content of paclitaxel in yew plants is generally very low, even if it is less than 0.1% in the bark of yew yew, which is currently recognized as having the highest content, but it is in great demand. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02C12N5/04
CPCC12P17/02C12N5/04C12N2500/05C12N2500/76
Inventor 马丹黄友法
Owner 天津市博爱生物药业有限公司
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