Culture medium for high-density culture of taxus chinensis cells

Abstract

The invention provides a culture medium for high-density culture of taxus chinensis cells. According to the culture medium, sodium nitrate and dipotassium phosphate serve as a nitrogen source and a phosphorus source respectively, certain calcium chloride is added, and on the basis of the calcium chloride, it is accidently found out that it is helpful to increase cell culture density by adding a certain type of traditional Chinese medicine of certain ingredients; based on the mentioned beneficial finding, traditional Chinese medicine ingredients, such as gymnadenia cono-psea, hibiscus rosa-sinensis, streptocaulon griffithii hook., cassia oil, sensitive plants, hawthorn, coriander seeds, anectochilus taivcanensis hayata, pyropolyporus fomentarius teng which are originally used for human disease treatment are selected preferably through an experiment means, the obtained culture medium accidently obtains an outstanding culture effect, the culture density of the taxus chinensis cells is remarkably increased, and meanwhile the growth speed is higher. According to the culture medium, a superior technical effect is obtained through an innovative technical means, meanwhile, the cost is low, and the culture medium is easy to implement and thus has good application prospect.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a medium for high-density culture of yew cells. Background technique [0002] Yew, also known as yew, also known as Chinese yew, is a national first-class rare and protected tree species in China, and is also recognized as an endangered natural rare anti-cancer plant in the world. [0003] Paclitaxel and its analogues only exist in the genus Taxus and Australian Taxus in the gymnosperm Taxaceae. There are 11 species of Taxus in the world, which are widely distributed in the frigid, temperate and subtropical regions of Eurasia and North America. area, but is scattered and slow-growing. Taxus genus Australia is only distributed in the southern hemisphere, and its quantity is rare. The content of paclitaxel in yew plants is generally very low, even if it is less than 0.1% in the bark of yew yew, which is currently recognized as having the highest content, but it is in great de...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a medium for high-density cultivation of yew cells, so as to solve the technical problem of the low density of plant cell culture medium for yew cells in the prior art