A method for removing cyanide from compound fermented rubber seeds

A technology of composite fermentation and fermented rubber, applied in the biological field, to achieve the effect of inhibiting the growth of bacteria, good removal effect and guaranteed safety

Active Publication Date: 2019-09-27
贵州中科微农生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are few studies on the removal of cyanide by the compound fermentation of existing microorganisms. The purpose of the present invention is to provide a method for removing cyanide from rubber seeds by mixed fermentation of Pichia kuroshii and fusion Weissella

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the method for removing cyanide by mixing fermented rubber seeds of Pichia kuroshii and fusion Weissella, the specific operations are as follows:

[0014] (1) Inoculate the purified and frozen Pichia kruorii into 5mL YPD liquid medium at a rate of 5% by volume and culture on a shaker at 30°C for 18h until the number of effective viable bacteria is ≥10 10 CFU / mL; inoculate 5 mL of MRS liquid medium with 5% inoculum volume of purified Weissella fused and preserved in cryopreservation, and culture on a shaker at 37°C for 16 hours until the number of effective viable bacteria is ≥10 10 CFU / mL;

[0015] (2) After mixing the activated Pichia kuruii and the fusion Weissella at a volume ratio of 1:1, inoculate the mixed strains in the fermentation medium at a ratio of 5% by volume, at 25 Under the condition of ℃, stand for fermentation and culture for 144 hours, wherein the fermentation medium is composed of water and pulverized rubber seeds passed through a 20-m...

Embodiment 2

[0018] Embodiment 2: the method for removing cyanide of this composite fermented rubber seed, concrete operations are as follows:

[0019] (1) Inoculate the purified and frozen Pichia kuroi into 5mL YPD liquid medium at 32°C for 20 hours on a shaker at a volume percentage of 5%, until the number of effective viable bacteria is ≥10 10 CFU / mL; Inoculate the purified Weissella fused with 5% inoculum into 5mL MRS liquid medium, culture on a shaker at 35°C for 20h, until the number of effective viable bacteria is ≥10 10 CFU / mL;

[0020] (2) After mixing the activated Pichia kurui and Weissella fusion at a volume ratio of 2:1, inoculate the mixed strains in the fermentation medium at a ratio of 8% by volume, at 30 Cultivate statically for 144 hours under the condition of ℃, in which the components of the fermentation medium are water and pulverized rubber seeds passed through a 40-mesh sieve, the ratio of the two is 1:2, and the fermented rubber seeds from which cyanide is removed ...

Embodiment 3

[0023] Embodiment 3: the method for removing cyanide of this composite fermented rubber seed, concrete operations are as follows:

[0024] (1) Inoculate the purified and frozen Pichia kushori at a volume percentage of 5% in 5mL YPD liquid medium for 24 hours on a shaker at 30°C until the number of effective viable bacteria is ≥10 10 CFU / mL; Inoculate the purified Weissella fused with 5% inoculum into 5mL MRS liquid medium, and culture on a shaking table at 30°C for 24h until the number of effective viable bacteria is ≥10 10 CFU / mL;

[0025] (2) After mixing the activated Pichia kurui and Weissella fusion at a volume ratio of 1:2, inoculate the mixed strains in the fermentation medium at a ratio of 10% by volume, at 37 It is obtained by static culture at ℃ for 120 hours, in which the fermentation medium is water and rubber seed powder crushed to about 20 mesh and passed through a 60 mesh sieve, the ratio of the two is 2:1, and the fermented rubber from cyanide is obtained afte...

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PUM

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Abstract

The invention discloses a method for removing cyanide from compound fermented rubber seeds. The method adopts Pichia pastoris ( Pichia kudriavzevii ) and Weissella fusion ( Weissella confusa ) A method for removing cyanide from compound fermented rubber seeds. In this method, Pichia kurcheni and Weissella fusion are respectively activated and cultured until the number of effective viable bacteria is ≥10 10 CFU / mL, mixed and added to a culture medium mixed with water and crushed rubber seed kernels for culture; research has found that compound fermentation has a very good effect on cyanide removal, with cyanide removal rates above 88.9%. . Acid production by lactobacilli can inhibit the growth of miscellaneous bacteria, while yeast fermentation increases flavor and effectively removes cyanide without deteriorating the rubber seeds.

Description

technical field [0001] The invention relates to a method for removing cyanide from rubber seeds compoundly fermented by Pichia kushori and Weissella fusion, belonging to the field of biotechnology. Background technique [0002] rubber( Hevea brasiliensis ) Euphorbiaceae trees, up to 30 meters high, rich in latex. Rubber tree is a typical tropical crop, native to Brazil; it is now widely cultivated in tropical Asia. It is cultivated in Hekou, Xishuangbanna and Ruili in Yunnan; it is cultivated in Guangdong, Guangxi, Hainan, southern Fujian and Taiwan. Capsule elliptic, 5-6 cm in diameter, with 3 longitudinal grooves, with a beak tip at the top, slightly concave at the base, thin exocarp, reticular veins at the bottom, thick endocarp, woody; seeds elliptic, light gray brown, There are markings. Rubber seeds are by-products of the rubber tree. For a long time in the past, a large number of rubber seeds have not been rationally and comprehensively utilized, and have been di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/37A23K10/12C12N1/16C12N1/20C12R1/84C12R1/01
CPCC12N1/16C12N1/20Y02P60/87
Inventor 邓先余陈晗门文卉李先永林连兵郭军
Owner 贵州中科微农生物技术有限公司
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