Library construction method for whole genome methylation bisulfite sequencing
A technology for methylation bisulfite and library construction, which can be used in chemical libraries, biochemical equipment and methods, combinatorial chemistry, etc., and can solve problems such as ineffective application
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0080] Example 1 Using the method of the present invention to construct a library for whole-genome methylation bisulfite sequencing
[0081] (1) gDNA fragmentation: the initial amount of 100ng genomic DNA was fragmented by an ultrasonic interrupter. Ultrasonic interruption was used to fragment gDNA for 30 seconds, stop for 30 seconds, and repeat 22 times. The interrupted product was purified by 1.8 times Ampure magnetic beads, 20 μL ddH 2 O eluted.
[0082] (2) Bisulfite treatment: EZDNAMethylation-GoldKit (manufacturer Zymo) was used to treat the product in the previous step with Bisulfite and eluted with 31 μLEB.
[0083] (3) One-strand synthesis: react according to the system in the table below. After mixing the first 4 reagents, put them at 65°C for 3 minutes, then drop to 4°C, add 4μL NEBKlenowexo-(28U / μL), and execute procedure 4. Incubate for 5 minutes at ℃, increase the temperature by 0.5 ℃ every 6 seconds until the temperature reaches 37 ℃, and then react at 37 ℃ for 60 minu...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com