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Artificial cultivation method for sporocarp of bacterial strain of agaricus bisporus

A bisporus mushroom, artificial cultivation technology, applied in botany equipment and methods, fertilizer mixture, gardening, etc., can solve the problems of high site and environmental requirements, long cultivation period, and unsatisfactory conditions, and achieve low environmental requirements and fruiting The effect of wide temperature range and simple operation

Inactive Publication Date: 2016-02-03
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the artificial culture methods of Agaricus bisporus fruit bodies are relatively mature. However, most of these methods are cultivated on composted and fermented compost and covered with soil. The cultivation period is long, the requirements for site and environment are high, and the management is cumbersome.
It cannot meet the requirements of scientific experiments such as breeding, quality analysis, differentiation and development that require artificial cultivation in a short period of time to obtain fruiting bodies.

Method used

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  • Artificial cultivation method for sporocarp of bacterial strain of agaricus bisporus
  • Artificial cultivation method for sporocarp of bacterial strain of agaricus bisporus
  • Artificial cultivation method for sporocarp of bacterial strain of agaricus bisporus

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1 Agaricus bisporus G1 bacterial strain is cultivated on the basal medium and obtains the method for fruiting body

[0029] (1) Basal medium formula: glucose 20g, peptone 2g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, vitamin B 1 10mg, agar 20g, distilled water 1000mL.

[0030] (2) Preparation of basal medium plate: Accurately weigh each component according to the medium formula, and dissolve each component in 900 mL of distilled water in turn. After the ingredients are completely dissolved, add water to 1000mL. Divide into Erlenmeyer flasks, add cotton plugs, bandage, sterilize at 121.3°C for 20 minutes, wait for the medium to drop to about 60°C, pour the medium into sterile petri dishes, 20mL per dish, and make plates after solidification .

[0031] (3) Inoculation and culture method: Take out the plate strains of G1 strain and As2796 strain from the refrigerator, use a hole punch with an inner diameter of 5mm to take the bacterial cake a...

Embodiment 2

[0032] Example 2 The method for culturing the Agaricus bisporus G1 strain to obtain fruiting bodies on the wheat grain boiling juice medium

[0033] (1) Formula of wheat grain boiling juice medium: 200 g of wheat grain (dry), 20 g of maltose, 2 g of peptone, 1.5 g of magnesium sulfate, 1 g of potassium dihydrogen phosphate, 20 g of agar, and 1000 mL of distilled water.

[0034] (2) Production of barley juice medium plate: Weigh 200g of dry wheat, wash it, boil it in water for 20 minutes, take its filtrate, make up water to 1000mL, add the rest of the ingredients in the formula to dissolve completely, and make the plate The method is the same as the preparation method of the above-mentioned basal medium.

[0035] (3) Inoculation and culture: Take out the G1 strain and the control strain As2796 plate strains from the refrigerator, use a puncher with an inner diameter of 5 mm to take the bacterial cake at the edge of the colony, and insert it into the center of the plate of the w...

Embodiment 3

[0036] Example 3 The method for culturing Agaricus bisporus G1 strain on wheat grain medium to obtain fruiting bodies

[0037] (1) The formula of wheat grain medium: wheat grain (dry) 98.5%, gypsum 1.5%.

[0038] (2) Preparation of wheat grain culture medium: Weigh 500g dry wheat grains, wash them, put them into boiled water, stir continuously, after boiling for 30min, filter with four layers of gauze, spread the wet wheat grains on a clean table, Cool at room temperature until there is no moisture on the surface of the wheat grains. At this time, the water content is about 40%. Collect the wheat grains, add 7.5g of gypsum, stir evenly, and divide them into 500mL can bottles, each with a capacity of 100g. Seal with polypropylene plastic film, 121.3 After sterilizing at ℃ for 1 hour, take it out and set aside.

[0039] (3) Inoculation and cultivation: Take out the G1 strain and the control strain As2796 strain from the refrigerator, take 1 cm × 1 cm sized bacterial block into ...

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Abstract

The invention provides an artificial cultivation method for a sporocarp of a bacterial strain of agaricus bisporus. According to the artificial cultivation method, the bacterial strain G1 of agaricus bisporus is adopted; the bacterial strain G1 is obtained through collection, separation and purification in Taiyuan City, Shanxi Province, and is preserved in the microbiological lab in the College of Life Science of Shanxi University. The bacterial strain G1 is inoculated on a basic medium, a kernel boiled juice culture medium or a kernel culture medium to obtain the sporocarp of the bacterial strain G1. Compared with the conventional artificial cultivation method of agaricus bisporus, the method provided by the invention is simple to operate, and low in field and environmental requirements, and has the advantage that the sporocarp can be obtained via artificial cultivation in a short time. The bacterial strain G1 is high in fruiting capability, and wide in fruiting temperature range (16 to 26 DEG C). The artificial cultivation method has an important meaning in developing wild genetic resource of agaricus bisporus, enriching the Chinese agaricus bisporus variety, and promoting agaricus bisporus breeding.

Description

technical field [0001] The invention relates to a method for cultivating edible fungi, in particular to a method for artificially cultivating fruit bodies of wild Agaricus bisporus. Background technique [0002] Agaricus bisporus (Lange) Imbach belongs to the order Agaricaceae, Agaricaceae, and the genus Agaricus bisporus (Lange) Imbach in classification. Also known as mushrooms, mushrooms for short. It is delicious, nutritious, and has high edible and medicinal value. It is currently the world's largest edible fungus. Its artificial cultivation began in the era of Louis XIV in France. [0003] At present, the artificial culture methods of Agaricus bisporus fruit bodies are relatively mature. However, most of these methods are cultivated and covered with soil on composted and fermented compost, which has a long cultivation period, high requirements on the site and environment, and cumbersome management. It cannot meet the requirements of some scientific experiments such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04
Inventor 康曼韩建荣张倢翟飞红
Owner SHANXI UNIV
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