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Method for quickly identifying sex of young daphnia of daphnia magna

A large-scale and fast technology, applied in special data processing applications, instruments, electrical and digital data processing, etc., can solve the problems of large workload and long time in the identification of females and males of newborn juveniles, reduce manual workload and solve identification problems. The effect of large workload and reduced test cost

Inactive Publication Date: 2016-02-03
YANGTZE UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for quickly identifying the sex of Daphnia large daphnia juveniles. This method solves the problem of heavy workload and long time-consuming in the identification of newborn juvenile Daphnia females and males, and achieves the goal of reducing manual workload and reducing testing costs. Purpose, can be used to quickly count the number of female and male juvenile daphnia

Method used

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  • Method for quickly identifying sex of young daphnia of daphnia magna
  • Method for quickly identifying sex of young daphnia of daphnia magna
  • Method for quickly identifying sex of young daphnia of daphnia magna

Examples

Experimental program
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Embodiment 1

[0024]A method for quickly identifying the sex of Daphnia magna juveniles, comprising the following steps:

[0025] 1) The three test tubes 4 on the left of the simple induction device were placed with 10 newborn male daphnia induced within 24 hours at 4°C, and the three test tubes 4 on the right were respectively placed with 10 newborn female daphnia induced within 24 hours at 4°C. Add 16.5cm-high GFID medium into the test tube, and adapt the newborn daphnia in the GFID medium for 1 hour. The part 2.5 cm above the bottom of test tube 4 is called system B, and the part 14 cm below the liquid surface of test tube 4 is called system A, where GFID The medium includes NaHCO with concentrations of 192mg / L, 120mg / L, 120mg / L and 8mg / L respectively 3 , CaSO 4 2H 2 O, MgSO 4 , KCl, its pH value and hardness are 7.6~8.0, 160~180mgCaCO 3 / L;

[0026] 2) Place the test tube 4 in step 1) on the test tube rack 2 of the induction simple device, and place the induction simple device unde...

Embodiment 2

[0030] A method for quickly identifying the sex of Daphnia magna juveniles, comprising the following steps:

[0031] 1) The three test tubes 4 on the left of the simple induction device are respectively placed with 5 newborn male daphnia induced at 4°C within 24 hours and 5 newborn female daphnia induced at 4°C within 24 hours. Add 16.5cm-high GFID medium into test tube 4, and adapt the newborn daphnia in GFID medium for 1 hour. The part 2.5 cm above the bottom of test tube 4 is called system B, and the part 14 cm below the liquid surface of test tube 4 is called system A. GFID medium includes NaHCO at concentrations of 192mg / L, 120mg / L, 120mg / L, 8mg / L, respectively 3 , CaSO 4 2H 2 O, MgSO 4 , KCl, its pH value and hardness are 7.6~8.0, 160~180mgCaCO 3 / L;

[0032] 2) Place the test tube 4 in step 1) on the test tube rack 2 of the induction simple device, and place the induction simple device under the light intensity of 30 μE / (m 2 .s), placed at 20°C for 24 hours;

[0...

Embodiment 3

[0036] A method for quickly identifying the sex of Daphnia magna juveniles, comprising the following steps:

[0037] 1) In the three test tubes 4 on the left of the simple induction device, 10 newborn male daphnia juveniles induced by fracturing flowback fluid within 24 hours were placed respectively, and in the three test tubes 4 on the right were placed respectively 10 newborn male daphnia juveniles induced by fracturing flowback fluid within 24 hours. Newborn female juvenile daphnia. Add 16.5cm-high GFID medium into the test tube, and the newborns adapt to the GFID medium for 1 hour. The part 2.5cm above the bottom of test tube 4 is called system B, and the part 14cm below the liquid surface of test tube 4 is called system A, where GFID The medium includes NaHCO with concentrations of 192mg / L, 120mg / L, 120mg / L and 8mg / L respectively 3 , CaSO 4 2H 2 O, MgSO 4 , KCl, its pH value and hardness are 7.6~8.0, 160~180mgCaCO 3 / L;

[0038] 2) Place the test tube 4 in step 1) ...

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Abstract

The invention discloses a method for quickly identifying sex of young daphnia of daphnia magna. The method comprises the steps of: putting newborn young daphnia in a test tube of a simple induction device; adding a GFID culture medium which is 16.5 cm high into the test tube, wherein the newborn young daphnia is adaptive in the GFID culture medium for 1 hour, the part which is 2.5 cm above the bottom of the test tube is called a system B, and the part which is 14 cm below a liquid level of the test tube is called a system A; and putting the induction simple device for 24 hours under the condition that the light density is 30 [Mu]E / (m2.s) and the temperature is 20 DEG C; after putting the device in a dark environment for 5 minutes, performing statistics on male daphnia and female daphnia entering into the system A or the system B at every 1 minute, respectively; and counting for 20 minutes and accumulating the quantity of the male daphnia and female daphnia entering into the system A or the system B in the time interval, wherein the result is that all the female daphnia enter into the system A while all the male daphnia enter into the system B. The method disclosed by the invention can be used for quickly identifying the sex of the newborn young daphnia and solves the problem that sex identification of newborn young daphnia is great in workload and overlong in consumed time.

Description

technical field [0001] The invention relates to an ecotoxicological test for Daphnia magna, in particular to a method for quickly identifying the sex of young Daphnia magna. Background technique [0002] Ecotoxicological testing techniques have been widely used in the ecological risk assessment of old and new chemicals. With the implementation of EU REACH regulations, the requirements for the ecological safety of chemicals entering the EU are becoming more and more stringent. my country's chemical product exports are facing challenges. Rigorous ecotoxicology and safety evaluation of chemicals is the trend of my country's chemical products. The premise of internationalization, so ecotoxicology and safety evaluation technology is particularly important, and the requirements for its technical level are getting higher and higher. The Chemical Registration Center of the State Environmental Protection Administration compiled the "Chemical Test Methods" to standardize and standardi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033G06F19/00
CPCA01K67/033G16Z99/00
Inventor 贺美许玫英梅承芳高亮
Owner YANGTZE UNIVERSITY
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