A kind of medicine for protecting stem cell therapy and preparation method thereof
A stem cell and drug technology, applied in the field of drugs for protecting stem cells and their preparation, can solve problems such as pulmonary ventilation stop, pulmonary arterial hypertension, hypoxia in patients, etc., and achieve inhibition of vasoconstriction, promotion of new blood vessel formation, and reduction of osmotic diuresis. Effect
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[0031] The preparation method of the medicine for protecting stem cell therapy, comprises the following steps:
[0032] (1), take raw materials carboxymethyl chitosan, dextran, prostaglandin E1, leukemia inhibitory factor, VEGF-A, and prepare PBS buffer solution, for subsequent use;
[0033](2), first heating the PBS damping fluid that step (1) obtains, then add carboxymethyl chitosan, dextran, VEGF-A, prostaglandin E1 and leukemia inhibitory factor successively, make respective mass-volume concentration reach respectively 1.2% to 2%, 0.5% to 0.8%, 0.2% to 0.4%, 0.1% to 0.2% and 1% to 1.5%, stir to obtain a mixed solution, and set aside;
[0034] (3), add medicinal active carbon in the mixed liquor that step (2) obtains, after stirring, filter and decarburize while hot, collect filtrate, for subsequent use;
[0035] (4), pH adjustment is carried out to the filtrate collected in step (3), the pH is adjusted to be between 7.2~7.4, then carry out primary filtration, the medicine...
Embodiment 1
[0047] A medicine for protecting stem cells, which is prepared by dissolving carboxymethyl chitosan, dextran, prostaglandin E1, leukemia inhibitory factor, VEGF-A in PBS buffer, wherein carboxymethyl chitosan, dextran The mass-volume concentrations of glycosides, VEGF-A, prostaglandin E1, and leukemia inhibitory factor are carboxymethyl chitosan 2%, dextran 0.5%, VEGF-A 0.2%, prostaglandin E1 0.1%, and leukemia inhibitory factor 1 %.
[0048] The preparation method of the medicine for protecting stem cell therapy comprises the following steps:
[0049] (1), get the raw material carboxymethyl chitosan, dextran, prostaglandin E1, leukemia inhibitory factor, VEGF-A and PBS buffer, for subsequent use;
[0050] (2), first heat the PBS buffer that step (1) obtains to 90 ℃, then add carboxymethyl chitosan, dextran, VEGF-A, prostaglandin E1 and leukemia inhibitory factor successively, make respective mass-volume Concentration reaches formula quantity respectively, obtains mixed liqu...
Embodiment 2
[0054] A medicine for protecting stem cells, which is prepared by dissolving carboxymethyl chitosan, dextran, prostaglandin E1, leukemia inhibitory factor, VEGF-A in PBS buffer, wherein carboxymethyl chitosan, dextran The mass-volume concentrations of glycosides, VEGF-A, prostaglandin E1, and leukemia inhibitory factor are carboxymethyl chitosan 1.2%, dextran 0.8%, VEGF-A 0.4%, prostaglandin E1 0.2%, and leukemia inhibitory factor 1.5 %.
[0055] The preparation method of the medicine for protecting stem cell therapy comprises the following steps:
[0056] Step (1), taking raw materials carboxymethyl chitosan, dextran, prostaglandin E1, leukemia inhibitory factor, VEGF-A and PBS buffer, for subsequent use;
[0057] Step (2), first heat the PBS buffer solution that step (1) obtains to 100 ℃, then add carboxymethyl chitosan, dextran, VEGF-A, prostaglandin E1 and leukemia inhibitory factor successively, make respective quality- When the volume concentration reaches the formula ...
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